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AB222905

Anti-MDMX/MDM4 antibody

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(3 Publications)

Rabbit Polyclonal MDMX/MDM4 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDM4 aa 200-350.

View Alternative Names

MDMX, MDM4, Protein Mdm4, Double minute 4 protein, Mdm2-like p53-binding protein, Protein Mdmx, p53-binding protein Mdm4

1 Images
Western blot - Anti-MDMX/MDM4 antibody (AB222905)
  • WB

Supplier Data

Western blot - Anti-MDMX/MDM4 antibody (AB222905)

All lanes:

Western blot - Anti-MDMX/MDM4 antibody (ab222905) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

Mouse stomach tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/500000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human MDM4 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

O15151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MDMX protein also known as MDM4 is an important regulator in the control of the p53 tumor suppressor pathway. Typically MDMX can be found expressed in multiple tissue types including the brain and kidney. MDMX with a molecular mass of approximately 54 kDa functions as an E3 ligase that binds to the p53 protein inhibiting its transcriptional activity and promoting its degradation. This precise interaction with p53 allows the regulation of cell growth and apoptosis making MDMX an important target for cancer research.
Biological function summary

MDMX contributes significantly to cell cycle regulation and apoptosis by forming a complex with MDM2 another E3 ubiquitin ligase. Together with MDM2 MDMX modulates the stability and activity of p53 therefore influencing cell fate decisions. This complex is important in preventing unwarranted p53 activity ensuring that the cell only undergoes apoptosis or cell cycle arrest in response to specific stress signals.

Pathways

The MDMX protein plays a central role in the p53 signaling pathway a critical mechanism in cellular response to genomic damage. It interacts directly with MDM2 to regulate p53's activity maintaining a balance between cell proliferation and death. This pathway is tightly linked to the DNA damage response providing a checkpoint during the cell cycle to prevent the propagation of genetically unstable cells. Additionally MDMX influences the PI3K/AKT pathway further intertwining its function with cellular survival processes.

The deregulation of MDMX is often associated with various forms of cancer including breast and colorectal cancers. Overexpression or genetic amplification of MDMX can lead to impaired p53 activity which promotes cell survival and proliferation in the presence of DNA damage. MDMX has a close connection with MDM2 in these disease contexts as both proteins synergize to inhibit p53 contributing to tumorigenesis. Therapeutic targeting of MDMX and its interaction with MDM2 offers a potential strategy for reinstating p53 activity in cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Along with MDM2, contributes to TP53 regulation (PubMed : 32300648). Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
See full target information MDM4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Animal nutrition (Zhongguo xu mu shou yi xue hui) 13:282-288 PubMed37168450

2023

Dietary adenosine supplementation improves placental angiogenesis in IUGR piglets by up-regulating adenosine A2a receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Zifang Wu,Jiawei Nie,Deyuan Wu,Shuangbo Huang,Jianzhao Chen,Huajin Liang,Xiangyu Hao,Li Feng,Hefeng Luo,Chengquan Tan

Respiratory research 23:340 PubMed36496404

2022

Involvement of miRNA-34a regulated Krüppel-like factor 4 expression in hyperoxia-induced senescence in lung epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hajime Maeda,Hongwei Yao,Hayato Go,Kelsey E Huntington,Monique E De Paepe,Phyllis A Dennery

Cancer medicine 9:9138-9149 PubMed33146951

2020

LncRNA MALAT1 accelerates non-small cell lung cancer progression via regulating miR-185-5p/MDM4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Suhong Zhang,Min Zhao,Fengling Chen
View all publications
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