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AB243859

Anti-MDMX/MDM4 antibody [BL-3-2F2]

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(1 Review)

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(2 Publications)

Rabbit Recombinant Monoclonal MDMX/MDM4 antibody. Suitable for IP, WB, Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MDM4 aa 100 to C-terminus.

View Alternative Names

MDMX, MDM4, Protein Mdm4, Double minute 4 protein, Mdm2-like p53-binding protein, Protein Mdmx, p53-binding protein Mdm4

4 Images
Flow Cytometry - Anti-MDMX/MDM4 antibody [BL-3-2F2] (AB243859)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MDMX/MDM4 antibody [BL-3-2F2] (AB243859)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling MDMX/MDM4 with ab243859 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details, Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

Immunoprecipitation - Anti-MDMX/MDM4 antibody [BL-3-2F2] (AB243859)
  • IP

Unknown

Immunoprecipitation - Anti-MDMX/MDM4 antibody [BL-3-2F2] (AB243859)

MDMX/MDM4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab243859 at 20μg/mL. Western blot was performed from the immunoprecipitate using ab243859 at 1/100 dilution.

Lane 1 : ab243859 IP in Jurkat whole cell lysate.
Lane 2 : Control IgG IP.

Chemiluminescence with an exposure time of 75 seconds.

All lanes:

Immunoprecipitation - Anti-MDMX/MDM4 antibody [BL-3-2F2] (ab243859)

Predicted band size: 55 kDa

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Western blot - Anti-MDMX/MDM4 antibody [BL-3-2F2] (AB243859)
  • WB

Supplier Data

Western blot - Anti-MDMX/MDM4 antibody [BL-3-2F2] (AB243859)

All lanes:

Western blot - Anti-MDMX/MDM4 antibody [BL-3-2F2] (ab243859) at 1/1000 dilution

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

MCF-7 whole cell lysate at 50 µg

Lane 3:

RKO whole cell lysate at 50 µg

Lane 4:

U2OS whole cell lysate at 50 µg

Lane 5:

HEK293T whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 54 kDa

Observed band size: 70 kDa

true

Exposure time: 75s

Western blot - Anti-MDMX/MDM4 antibody [BL-3-2F2] (AB243859)
  • WB

Unknown

Western blot - Anti-MDMX/MDM4 antibody [BL-3-2F2] (AB243859)

All lanes:

Western blot - Anti-MDMX/MDM4 antibody [BL-3-2F2] (ab243859)

Lane 1:

Jurkat cell lysate

Lane 2:

293T cell lysate

Lane 3:

HeLa cell lysate

Lane 4:

A549 cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-3-2F2

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB, IP

applications

Immunogen

Synthetic Peptide within Human MDM4 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 20μg/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Cell culture supernatant was harvested from mammalian cells expressing recombinant rabbit monoclonal antibody.
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MDMX protein also known as MDM4 is an important regulator in the control of the p53 tumor suppressor pathway. Typically MDMX can be found expressed in multiple tissue types including the brain and kidney. MDMX with a molecular mass of approximately 54 kDa functions as an E3 ligase that binds to the p53 protein inhibiting its transcriptional activity and promoting its degradation. This precise interaction with p53 allows the regulation of cell growth and apoptosis making MDMX an important target for cancer research.
Biological function summary

MDMX contributes significantly to cell cycle regulation and apoptosis by forming a complex with MDM2 another E3 ubiquitin ligase. Together with MDM2 MDMX modulates the stability and activity of p53 therefore influencing cell fate decisions. This complex is important in preventing unwarranted p53 activity ensuring that the cell only undergoes apoptosis or cell cycle arrest in response to specific stress signals.

Pathways

The MDMX protein plays a central role in the p53 signaling pathway a critical mechanism in cellular response to genomic damage. It interacts directly with MDM2 to regulate p53's activity maintaining a balance between cell proliferation and death. This pathway is tightly linked to the DNA damage response providing a checkpoint during the cell cycle to prevent the propagation of genetically unstable cells. Additionally MDMX influences the PI3K/AKT pathway further intertwining its function with cellular survival processes.

The deregulation of MDMX is often associated with various forms of cancer including breast and colorectal cancers. Overexpression or genetic amplification of MDMX can lead to impaired p53 activity which promotes cell survival and proliferation in the presence of DNA damage. MDMX has a close connection with MDM2 in these disease contexts as both proteins synergize to inhibit p53 contributing to tumorigenesis. Therapeutic targeting of MDMX and its interaction with MDM2 offers a potential strategy for reinstating p53 activity in cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to p53/TP53 regulation (PubMed : 32300648). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding their transcriptional activation domains. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
See full target information MDM4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar

JCI insight 7: PubMed35801592

2022

Milademetan is a highly potent MDM2 inhibitor in Merkel cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Varsha Ananthapadmanabhan,Thomas C Frost,Kara M Soroko,Aine Knott,Brianna J Magliozzi,Prafulla C Gokhale,Vijaya G Tirunagaru,Robert C Doebele,James A DeCaprio
View all publications
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Product promise

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