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AB313615

Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free

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Rabbit Recombinant Monoclonal MDMX/MDM4 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

MDMX, MDM4, Protein Mdm4, Double minute 4 protein, Mdm2-like p53-binding protein, Protein Mdmx, p53-binding protein Mdm4

4 Images
Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free (AB313615)
  • WB

Supplier Data

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free (AB313615)

This data was developed using ab313614, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The identity of the lower MW band at approximately 60 kDa may represent an N-terminal truncated isoform of MDMX/MDM4 (PMID : 25659040). This has not been confirmed with additional tests. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 3 minutes

All lanes:

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] (<a href='/en-us/products/primary-antibodies/mdmx-mdm4-antibody-epr25209-54-ab313614'>ab313614</a>) at 1/1000 dilution

Lane 1:

HCT116 (human colorectal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HCT116 transfected with siRNA specifically targeting MDMX/MDM4 whole cell lysate at 20 µg

Lane 3:

HCT116 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 3min

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free (AB313615)
  • WB

Supplier Data

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free (AB313615)

This data was developed using ab313614, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation Exposure time : 114 seconds

All lanes:

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] (<a href='/en-us/products/primary-antibodies/mdmx-mdm4-antibody-epr25209-54-ab313614'>ab313614</a>) at 1/1000 dilution

All lanes:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 114s

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free (AB313615)
  • WB

Supplier Data

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free (AB313615)

This data was developed using ab313614, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 158 seconds

All lanes:

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] (<a href='/en-us/products/primary-antibodies/mdmx-mdm4-antibody-epr25209-54-ab313614'>ab313614</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 158s

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free (AB313615)
  • WB

Supplier Data

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] - BSA and Azide free (AB313615)

This data was developed using ab313614, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : NCI-H1299 (PMID : 18172009)

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

The identity of the lower MW band at approximately 60 kDa may represent an N-terminal truncated isoform of MDMX/MDM4 (PMID : 25659040). This has not been confirmed with additional tests.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MDMX/MDM4 antibody [EPR25209-54] (<a href='/en-us/products/primary-antibodies/mdmx-mdm4-antibody-epr25209-54-ab313614'>ab313614</a>) at 1/1000 dilution

Lane 1:

JEG-3 (human placenta epithelial cell) whole cell lysate at 20 µg

Lane 2:

NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

true

Exposure time: 114s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25209-54

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The identity of the lower MW band at approximately 60 kDa may represent an N-terminal truncated isoform of MDMX/MDM4 (PMID: 25659040). This has not been confirmed with additional tests.

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Complementary Products

Primary Antibodies

AB259265

Anti-MDM2 antibody [EPR22256-98]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MDM2 antibody [EPR22256-98] (AB259265)

  • 5
  • 5
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Proteins & Peptides

AB167947

Recombinant Human MDMX/MDM4 protein

SDS-PAGE - Recombinant Human MDMX/MDM4 protein (AB167947)

  • 0
  • 0
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Reactivity data

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Product details

ab313615 is the carrier-free version of ab313614.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MDMX protein also known as MDM4 is an important regulator in the control of the p53 tumor suppressor pathway. Typically MDMX can be found expressed in multiple tissue types including the brain and kidney. MDMX with a molecular mass of approximately 54 kDa functions as an E3 ligase that binds to the p53 protein inhibiting its transcriptional activity and promoting its degradation. This precise interaction with p53 allows the regulation of cell growth and apoptosis making MDMX an important target for cancer research.
Biological function summary

MDMX contributes significantly to cell cycle regulation and apoptosis by forming a complex with MDM2 another E3 ubiquitin ligase. Together with MDM2 MDMX modulates the stability and activity of p53 therefore influencing cell fate decisions. This complex is important in preventing unwarranted p53 activity ensuring that the cell only undergoes apoptosis or cell cycle arrest in response to specific stress signals.

Pathways

The MDMX protein plays a central role in the p53 signaling pathway a critical mechanism in cellular response to genomic damage. It interacts directly with MDM2 to regulate p53's activity maintaining a balance between cell proliferation and death. This pathway is tightly linked to the DNA damage response providing a checkpoint during the cell cycle to prevent the propagation of genetically unstable cells. Additionally MDMX influences the PI3K/AKT pathway further intertwining its function with cellular survival processes.

The deregulation of MDMX is often associated with various forms of cancer including breast and colorectal cancers. Overexpression or genetic amplification of MDMX can lead to impaired p53 activity which promotes cell survival and proliferation in the presence of DNA damage. MDMX has a close connection with MDM2 in these disease contexts as both proteins synergize to inhibit p53 contributing to tumorigenesis. Therapeutic targeting of MDMX and its interaction with MDM2 offers a potential strategy for reinstating p53 activity in cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to p53/TP53 regulation (PubMed : 32300648). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding their transcriptional activation domains. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
See full target information MDM4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com