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AB122926

Anti-MDMX/MDM4 (phospho S367) antibody [15]

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(2 Publications)

Mouse Monoclonal MDMX/MDM4 phospho S367 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

MDMX, MDM4, Protein Mdm4, Double minute 4 protein, Mdm2-like p53-binding protein, Protein Mdmx, p53-binding protein Mdm4

1 Images
Western blot - Anti-MDMX/MDM4 (phospho S367) antibody [15] (AB122926)
  • WB

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Western blot - Anti-MDMX/MDM4 (phospho S367) antibody [15] (AB122926)

The upper lanes show incubation with a non-phosphos antibody to MDMX/MDM4. The lower lanes as indicated above.
The cell lysates were immunoprecipitated with another anti-MDMX/MDM4 antibody before western blotting.

All lanes:

Western blot - Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926) at 1 µg/mL

Lane 1:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM)

Lane 2:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 1 hour

Lane 3:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 2 hour

Lane 4:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 3 hour

Lane 5:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 4 hour

Lane 6:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 1 hour

Lane 7:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 2 hour

Lane 8:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 3 hour

Lane 9:

MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 4 hour

Predicted band size: 55 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

15

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MDMX protein also known as MDM4 is an important regulator in the control of the p53 tumor suppressor pathway. Typically MDMX can be found expressed in multiple tissue types including the brain and kidney. MDMX with a molecular mass of approximately 54 kDa functions as an E3 ligase that binds to the p53 protein inhibiting its transcriptional activity and promoting its degradation. This precise interaction with p53 allows the regulation of cell growth and apoptosis making MDMX an important target for cancer research.
Biological function summary

MDMX contributes significantly to cell cycle regulation and apoptosis by forming a complex with MDM2 another E3 ubiquitin ligase. Together with MDM2 MDMX modulates the stability and activity of p53 therefore influencing cell fate decisions. This complex is important in preventing unwarranted p53 activity ensuring that the cell only undergoes apoptosis or cell cycle arrest in response to specific stress signals.

Pathways

The MDMX protein plays a central role in the p53 signaling pathway a critical mechanism in cellular response to genomic damage. It interacts directly with MDM2 to regulate p53's activity maintaining a balance between cell proliferation and death. This pathway is tightly linked to the DNA damage response providing a checkpoint during the cell cycle to prevent the propagation of genetically unstable cells. Additionally MDMX influences the PI3K/AKT pathway further intertwining its function with cellular survival processes.

The deregulation of MDMX is often associated with various forms of cancer including breast and colorectal cancers. Overexpression or genetic amplification of MDMX can lead to impaired p53 activity which promotes cell survival and proliferation in the presence of DNA damage. MDMX has a close connection with MDM2 in these disease contexts as both proteins synergize to inhibit p53 contributing to tumorigenesis. Therapeutic targeting of MDMX and its interaction with MDM2 offers a potential strategy for reinstating p53 activity in cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Along with MDM2, contributes to TP53 regulation (PubMed : 32300648). Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
See full target information MDM4 phospho S367
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:9420-33 PubMed25826083

2015

PTPRO-mediated autophagy prevents hepatosteatosis and tumorigenesis.

Applications

WB

Species

Mouse

Wenjie Zhang,Jiajie Hou,Xiaochen Wang,Runqiu Jiang,Yin Yin,Jie Ji,Lei Deng,Xingxu Huang,Ke Wang,Beicheng Sun

Molecular and cellular biology 25:9608-20 PubMed16227609

2005

DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Okamoto,Kenji Kashima,Yaron Pereg,Michiko Ishida,Satomi Yamazaki,Ayumi Nota,Amina Teunisse,Domenico Migliorini,Issay Kitabayashi,Jean-Christophe Marine,Carol Prives,Yosef Shiloh,Aart G Jochemsen,Yoichi Taya
View all publications
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