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AB97445

Anti-ME1 antibody

4

(1 Review)

|

(31 Publications)

Rabbit Polyclonal ME1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 31 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ME1 aa 150-500.

View Alternative Names

NADP-dependent malic enzyme, NADP-ME, Malic enzyme 1, ME1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-ME1 antibody (AB97445)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ME1 antibody (AB97445)

Immunofluorescence analysis of ME1 in paraformaldehyde fixed HeLa, using ab97445 at a 1/200 dilution.
Lower image : merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME1 antibody (AB97445)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME1 antibody (AB97445)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue stained for ME1 protein at cytosol with ab97445 at a 1/500 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

Immunoprecipitation - Anti-ME1 antibody (AB97445)
  • IP

Supplier Data

Immunoprecipitation - Anti-ME1 antibody (AB97445)

Immunoprecipitation of ME1 protein from HeLa whole cell extracts using 5 µg of ab97445. Western blot analysis was performed using ab97445.

All lanes:

Immunoprecipitation - Anti-ME1 antibody (ab97445) at 5 µg

Lane 1:

HeLa whole cell extracts with control IgG

Lane 2:

HeLa whole cell extracts

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 64 kDa

false

Western blot - Anti-ME1 antibody (AB97445)
  • WB

Supplier Data

Western blot - Anti-ME1 antibody (AB97445)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ME1 antibody (ab97445) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 64 kDa

false

Western blot - Anti-ME1 antibody (AB97445)
  • WB

Supplier Data

Western blot - Anti-ME1 antibody (AB97445)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ME1 antibody (ab97445) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

Me1 shRNA transfected HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 64 kDa

false

Western blot - Anti-ME1 antibody (AB97445)
  • WB

Supplier Data

Western blot - Anti-ME1 antibody (AB97445)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ME1 antibody (ab97445) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 64 kDa

false

Western blot - Anti-ME1 antibody (AB97445)
  • WB

Supplier Data

Western blot - Anti-ME1 antibody (AB97445)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ME1 antibody (ab97445) at 1/1000 dilution

Lane 1:

NIH-3T3 whole cell lysates at 30 µg

Lane 2:

JC whole cell lysates at 30 µg

Lane 3:

BCL-1 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

ICC/IF, WB, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ME1 aa 150-500. The exact immunogen used to generate this antibody is proprietary information.

P48163

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ME1 also known as malic enzyme 1 is a cytosolic enzyme that catalyzes the reversible oxidative decarboxylation of malate to pyruvate generating NADPH in the process. This enzyme contributes to cellular lipid and cholesterol biosynthesis. ME1 has an approximate molecular weight of 64 kDa. You can find ME1 expression mainly in tissues with high biosynthetic activities such as liver fat and kidney.
Biological function summary

ME1 plays an important role in regulating the cellular redox state and maintaining metabolic homeostasis. It supplies NADPH which is necessary for fatty acid synthesis and lipid metabolism supporting anabolic processes in cells. ME1 works as a part of a metabolic enzyme complex that coordinates with other enzymes to produce reducing equivalents required for detoxification and biosynthesis. Scientists frequently study its role in lipid synthesis due to its implications in metabolic disorders.

Pathways

ME1 is involved in lipid metabolism and the pentose phosphate pathway where it helps generate reducing power. ME1 interacts with other enzymes like glucose-6-phosphate dehydrogenase which also provides NADPH showing its interconnected role in maintaining cellular redox balance. ME1’s activity supports both pathways by contributing to the production of ribose-5-phosphate and other precursors important for nucleotide biosynthesis and antioxidant defense.

ME1 has links to obesity and cancer where its dysregulation can promote tumorigenesis and metabolic disorders. Studies suggest aberrant ME1 expression may lead to altered lipid biosynthesis contributing to excessive fat accumulation in obesity. ME1 and glutamine synthetase a protein connected in cancer metabolism work together to support cell proliferation through their metabolic pathways highlighting ME1’s potential as a target in metabolic and cancer therapy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate.
See full target information ME1
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging 6:1621808 PubMed40636718

2025

The lifespan-extending MEK1 inhibitor trametinib promotes regulation of de novo lipogenesis enzymes by chaperone-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiexian Chen,Joshua Berg,Calvin M Burns,Hanyi Jia,Xinna Li,Richard A Miller,S Joseph Endicott,Gonzalo Garcia

Cancer & metabolism 13:14 PubMed40098185

2025

WTAP contributes to platinum resistance in high-grade serous ovarian cancer by up-regulating malic acid: insights from liquid chromatography and mass spectrometry analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Wang,Shuiqing Xu,Jianqing Xu,Jiahui Wei,Yumei Wu

Nature communications 15:8682 PubMed39375345

2024

Compensatory activity of the PC-ME1 metabolic axis underlies differential sensitivity to mitochondrial complex I inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Del Prado,Myriam Jaraíz-Rodríguez,Mauro Agro,Marcos Zamora-Dorta,Natalia Azpiazu,Manuel Calleja,Mario Lopez-Manzaneda,Jaime de Juan-Sanz,Alba Fernández-Rodrigo,José A Esteban,Mònica Girona,Albert Quintana,Eduardo Balsa

Biomedicines 12: PubMed39335602

2024

Transcriptional Regulation and Function of Malic Enzyme 1 in Human Macrophage Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Santarsiero,Simona Todisco,Paolo Convertini,Chiara De Leonibus,Vittoria Infantino

PloS one 19:e0303577 PubMed38843233

2024

Malic enzyme 1 knockout has no deleterious phenotype and is favored in the male germline under standard laboratory conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan M Alektiar,Mengrou Shan,Megan D Radyk,Li Zhang,Christopher J Halbrook,Lin Lin,Carlos Espinoza,Ivan F Mier,Brooke L Lavoie,Lucie Salvatore,Marina Pasca di Magliano,Lewis C Cantley,Jacob L Mueller,Costas A Lyssiotis

Journal of translational medicine 21:568 PubMed37620891

2023

ACLY as a modulator of liver cell functions and its role in Metabolic Dysfunction-Associated Steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Convertini,Anna Santarsiero,Simona Todisco,Michele Gilio,Donatella Palazzo,Ilaria Pappalardo,Dominga Iacobazzi,Maria Frontuto,Vittoria Infantino

Nature cardiovascular research 1:817-829 PubMed36776621

2022

Integrated landscape of cardiac metabolism in end-stage human nonischemic dilated cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Flam,Cholsoon Jang,Danielle Murashige,Yifan Yang,Michael P Morley,Sunhee Jung,Daniel S Kantner,Hannah Pepper,Kenneth C Bedi,Jeff Brandimarto,Benjamin L Prosser,Thomas Cappola,Nathaniel W Snyder,Joshua D Rabinowitz,Kenneth B Margulies,Zolt Arany

Clinical cancer research : an official journal of the American Association for Cancer Research 28:3573-3589 PubMed35421237

2022

Malic Enzyme 1 Absence in Synovial Sarcoma Shifts Antioxidant System Dependence and Increases Sensitivity to Ferroptosis Induction with ACXT-3102.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlyn B Brashears,Bethany C Prudner,Richa Rathore,Katharine E Caldwell,Carina A Dehner,Jane L Buchanan,Sara E S Lange,Neal Poulin,Jennifer K Sehn,Jason Roszik,Dirk Spitzer,Kevin B Jones,Regis O'Keefe,Torsten O Nielsen,Eric B Taylor,Jason M Held,William Hawkins,Brian A Van Tine

Redox biology 46:102065 PubMed34293554

2021

Lactate and glutamine support NADPH generation in cancer cells under glucose deprived conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Minfeng Ying,Duo You,Xiaobing Zhu,Limeng Cai,Siying Zeng,Xun Hu

Cell metabolism 33:1027-1041.e8 PubMed33770508

2021

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ping Wang,Azeem Sharda,Shuang-Nian Xu,Nick van Gastel,Cheuk Him Man,Una Choi,Wei Zhong Leong,Xi Li,David T Scadden
View all publications
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Product promise

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