Mouse Monoclonal NCAP antibody. Suitable for WB and reacts with Recombinant full length protein - Measles virus samples. Cited in 7 publications.
Images
![Western blot - Anti-Measles nucleoprotein antibody [3E1] (AB9397), expandable thumbnail](https://content.abcam.com/products/images/measles-nucleoprotein-antibody-3e1-ab9397--western-blot-img101223.gif "Western blot - Anti-Measles nucleoprotein antibody [3E1] (AB9397)")
Publications
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- International journal of molecular sciences 24:2023Novel Antiviral Molecules against Ebola Virus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMila Collados Rodríguez,Patrick Maillard,Alexandra Journeaux,Anastassia V Komarova,Valérie Najburg,Raul-Yusef Sanchez David,Olivier Helynck,Mingzhe Guo,Jin Zhong,Sylvain Baize,Frédéric Tangy,Yves Jacob,Hélène Munier-Lehmann,Eliane F MeursPubMed 37834238
- Nature communications 12:62772021A live measles-vectored COVID-19 vaccine induces strong immunity and protection from SARS-CoV-2 challenge in mice and hamsters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhanramphoei N Frantz,Aleksandr Barinov,Claude Ruffié,Chantal Combredet,Valérie Najburg,Guilherme Dias de Melo,Florence Larrous,Lauriane Kergoat,Samaporn Teeravechyan,Anan Jongkaewwattana,Emmanuelle Billon-Denis,Jean-Nicolas Tournier,Matthieu Prot,Laurine Levillayer,Laurine Conquet,Xavier Montagutelli,Magali Tichit,David Hardy,Priyanka Fernandes,Hélène Strick-Marchand,James Di Santo,Etienne Simon-Lorière,Hervé Bourhy,Frédéric TangyPubMed 34725327
- Cell host & microbe 16:19-302014Antagonism of the phosphatase PP1 by the measles virus V protein is required for innate immune escape of MDA5.Applications:WBReactive species:Unspecified reactive speciesMeredith E Davis,May K Wang,Linda J Rennick,Florian Full,Sebastian Gableske,Annelies W Mesman,Sonja I Gringhuis,Teunis B H Geijtenbeek,W Paul Duprex,Michaela U GackPubMed 25011105
- Journal of virology 87:9633-422013Measles virus nonstructural C protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinako Ito et. al.PubMed 23804634
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 29:90-1022013TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuanhong Sun,Bénédicte Sammut,Feng-Ming Wang,Noriyoshi Kurihara,Jolene J Windle,G David Roodman,Deborah L GalsonPubMed 23794264
- Bone 53:269-762012Measles virus nucleocapsid protein, a key contributor to Paget's disease, increases IL-6 expression via down-regulation of FoxO3/Sirt1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng-Ming Wang,Aliye Sarmasik,Yuko Hiruma,Quanhong Sun,Bénédicte Sammut,Jolene J Windle,G David Roodman,Deborah L GalsonPubMed 23262029
- Journal of virology 83:11645-542009Contribution of matrix, fusion, hemagglutinin, and large protein genes of the CAM-70 measles virus vaccine strain to efficient growth in chicken embryonic fibroblasts.Applications:WBReactive species:Unspecified reactive speciesLuna Bhatta Sharma et. al.PubMed 19726516
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant full length protein - Measles virus | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Measles virus | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases (By similarity). The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter (By similarity). The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis (By similarity). Forms the encapsidation complex with the phosphoprotein protein P (By similarity). Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P (By similarity). Participates, together with P, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place (By similarity). N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response (By similarity).
Additional Targets
N, N
Alternative names
NP, N, Nucleoprotein, Nucleocapsid protein, Protein N
Recommended products
Mouse Monoclonal NCAP antibody. Suitable for WB and reacts with Recombinant full length protein - Measles virus samples. Cited in 7 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 3E1
- Purification technique
- Affinity purification Protein A
- Specificity
Reacts with measles NP.
Does not cross-react with mumps NP.
Recognises both native and recombinant yeast-derived measles NP.- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR210126-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Measles nucleoprotein antibody [3E1] (ab9397)
Immunoblot of measles NP using monoclonal antibody 3E1.
Antibody 3E1 at a concentration of 0.5 μg/ml.
Lane 1 - prestained marker proteins
lane 2 - recombinant measles NP.
Immunoblot of measles NP using monoclonal antibody 3E1.
Antibody 3E1 at a concentration of 0.5 µg/ml.
Lane 1 - prestained marker proteins
lane 2 - recombinant measles NP.All lanes: Western blot - Anti-Measles nucleoprotein antibody [3E1] (ab9397)
Predicted band size: 58 kDa
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