Mouse Recombinant Monoclonal MECP2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat samples.
Images
![Western blot - Anti-MeCP2 antibody [4B4] - BSA and Azide free (AB255834), expandable thumbnail](https://content.abcam.com/products/images/mecp2-antibody-4b4-bsa-and-azide-free-ab255834--western-blot-img36966.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MeCP2 antibody [4B4] - BSA and Azide free (AB255834)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] - BSA and Azide free (AB255834), expandable thumbnail](https://content.abcam.com/products/images/mecp2-antibody-4b4-bsa-and-azide-free-ab255834--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118194.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] - BSA and Azide free (AB255834)")
Key facts
- Isotype
- IgG2c
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC | WB | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Additional Targets
Mecp2
Alternative names
Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, Mecp2
Recommended products
Mouse Recombinant Monoclonal MECP2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat samples.
Key facts
- Isotype
- IgG2c
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 4B4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255834 is the carrier-free version of Anti-MeCP2 antibody [4B4] ab252840.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834), expandable thumbnail](https://content.abcam.com/products/images/mecp2-antibody-4b4-bsa-and-azide-free-ab255834--western-blot-img36966.jpg "Western blot - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)")
Western blot - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MeCP2 antibody [4B4] ab252840).
All lanes: Western blot - Anti-MeCP2 antibody [4B4] (Anti-MeCP2 antibody [4B4] ab252840) at 1/1000 dilution
Lane 1: Rat brain cytoplasmic fraction at 20 µg
Lane 2: Rat brain nuclear fraction at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 78 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834), expandable thumbnail](https://content.abcam.com/products/images/mecp2-antibody-4b4-bsa-and-azide-free-ab255834--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118194.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MeCP2 with Anti-MeCP2 antibody [4B4] ab252840 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MeCP2 antibody [4B4] ab252840).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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