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AB277629

Anti-MeCP2 antibody [5H12]

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(2 Publications)

Mouse Monoclonal MECP2 antibody. Suitable for IHC, WB and reacts with Rat, Mouse, Cow samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MECP2.

View Alternative Names

Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2

2 Images
Immunohistochemistry - Anti-MeCP2 antibody [5H12] (AB277629)
  • IHC

Supplier Data

Immunohistochemistry - Anti-MeCP2 antibody [5H12] (AB277629)

Perfusion-fixed, free floating sections of rat cerebellum section stained for MeCP2 using ab277629 (red) at 1/1000 dilution, and counterstained with rabbit pAb to GFAP (green) at 1/5000 dilution in immunohistochemical analysis. The blue Hoechst is staining nuclear DNA. ab277629 specifically labels nuclei of neurons, while the GFAP antibody stains the network of astroglial cells and projections of Bergmann glia.

Western blot - Anti-MeCP2 antibody [5H12] (AB277629)
  • WB

Supplier Data

Western blot - Anti-MeCP2 antibody [5H12] (AB277629)

Lane 1:

Western blot - Anti-MeCP2 antibody [5H12] (ab277629)

Lanes 2 - 4:

Western blot - Anti-MeCP2 antibody [5H12] (ab277629) at 1/2000 dilution

Lane 2:

nuclear extract of rat brain

Lane 3:

nuclear extract of mouse brain

Lane 4:

cow cerebellum

Predicted band size: 52 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5H12

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse, Rat, Cow

Applications

IHC, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MECP2.

P51608

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Proteins & Peptides

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Primary Antibodies

AB32117

Anti-HDAC2 antibody [Y461]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "1/1000 - 1/5000", "IHC-species-notes": "<p>Transcardial perfusion-fixed with 4% paraformaldehyde for 1 hour, free floating sections.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from Tissue Culture supernatant.
Storage buffer
Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
See full target information MECP2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 5:103344 PubMed39331500

2024

Protocol for the neonatal intracerebroventricular delivery of adeno-associated viral vectors for brain restoration of MECP2 for Rett syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Kan Yang,Tianshu Li,Yixiao Geng,Ru Zhang,Zhankui Xu,Jun Wu,Yiting Yuan,Yuefang Zhang,Zilong Qiu,Fei Li

Sensors (Basel, Switzerland) 23: PubMed37112287

2023

Portable Surface Plasmon Resonance Detector for COVID-19 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Maciej Trzaskowski,Anna Mazurkiewicz-Pisarek,Jakub Waldemar Trzciński,Marcin Drozd,Rafał Podgórski,Anna Zabost,Ewa Augustynowicz-Kopeć
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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