Rabbit Polyclonal MECP2 antibody. Suitable for ChIP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MECP2 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Cell reports 42:1121482023DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loops.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEsther Marchena-Cruz,Lola P Camino,Jay Bhandari,Sónia Silva,José Javier Marqueta-Gracia,Shahad A Amdeen,Cristina Guillén-Mendoza,María L García-Rubio,José M Calderón-Montaño,Xiaoyu Xue,Rosa Luna,Andrés AguileraPubMed 36827184
- Journal of experimental & clinical cancer research 38:3662019A central role for MeCP2 in the epigenetic repression of miR-200c during epithelial-to-mesenchymal transition of glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErbao Bian et. al.PubMed 31429770
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MECP2 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P51608
Reactivity data
Select an application| ChIP | WB | |
|---|---|---|
| Human | Tested | Tested |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/cells for 6 µg/cells | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
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Target data
Function
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Alternative names
Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
Recommended products
Rabbit Polyclonal MECP2 antibody. Suitable for ChIP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MECP2 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MECP2 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P51608
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
ChIP - Anti-MeCP2 antibody - ChIP Grade (ab195393)
ChIP assays were performed using human osteosarcoma (U2OS) cells, ab195393 and optimized PCR primer sets. Sheared chromatin from 1x106 cells and 5 μg of antibody were used per ChIP experiment. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). Image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Western blot - Anti-MeCP2 antibody - ChIP Grade (ab195393)
TBS-Tween containing 5% skimmed milk
All lanes: Western blot - Anti-MeCP2 antibody - ChIP Grade (ab195393) at 1/1000 dilution
All lanes: nuclear extract from HeLa cells at 40 µg
Predicted band size: 52 kDa
Western blot - Anti-MeCP2 antibody - ChIP Grade (ab195393)
MeCP2 Western blot staining using rabbit Anti-MeCP2 antibody
Western blot: Rabbit Polyclonal to MeCP2 ab195393 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 70 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in MECP2 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes: Western blot - Anti-MeCP2 antibody - ChIP Grade (ab195393) at 1/1000 dilution
Lane 1: Wild-type U-87 MG at 20 µg
Lane 2: Western blot - Human MECP2 knockout U-87 MG cell line (ab306725) at 20 µg
Lane 3: Wild-type HAP1 at 20 µg
Lane 4: MECP2 knockout HAP1 at 20 µg
SecondaryAll lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 70 kDa
Western blot - Anti-MeCP2 antibody - ChIP Grade (ab195393)
Western blot: Rabbit Polyclonal to MeCP2 ab195393 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 52 kDa in Wild-type HCT 116 ab288559 cell lysates with no signal observed at this size in MECP2 knockout HCT 116 Human MECP2 knockout HCT116 cell line ab289193 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes: Western blot - Anti-MeCP2 antibody - ChIP Grade (ab195393) at 1/1000 dilution
Lane 1: Wild-type HCT 116 ab288559 at 20 µg
Lane 2: MECP2 knockout HCT 116 Human MECP2 knockout HCT116 cell line ab289193 at 20 µg
Lane 3: Wild-type HAP1 at 20 µg
Lane 4: MECP2 knockout HAP1 at 20 µg
SecondaryAll lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
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