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AB254050

Anti-MeCP2 (phospho S421) antibody

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(2 Publications)

Rabbit Polyclonal MECP2 phospho S421 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MECP2 phospho S421.

View Alternative Names

Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2

1 Images
Western blot - Anti-MeCP2 (phospho S421) antibody (AB254050)
  • WB

Supplier Data

Western blot - Anti-MeCP2 (phospho S421) antibody (AB254050)

All lanes:

Western blot - Anti-MeCP2 (phospho S421) antibody (ab254050) at 1/1000 dilution

Lane 1:

Mouse whole brain lysate

Lane 2:

Mouse whole brain lysate with immunizing peptide

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MECP2 phospho S421. The exact immunogen used to generate this antibody is proprietary information.

P51608

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.5 Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
See full target information MECP2 phospho S421
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Brain sciences 15: PubMed40149758

2025

The pCREB/BDNF Pathway in the Hippocampus Is Involved in the Therapeutic Effect of Selective 5-HT Reuptake Inhibitors in Adult Male Rats Exposed to Blast Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Fan,Hong Wang,Xiaoqiang Lv,Qi Wang,Boya Yu,Xiao Li,Liang Li,Yuhao Zhang,Ning Ma,Qing Lu,Airong Qian,Junhong Gao

Frontiers in pharmacology 13:972828 PubMed36147353

2022

Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2.

Applications

Unspecified application

Species

Unspecified reactive species

Baoyu Shen,Ruilin Zhang,Genmeng Yang,Yanxia Peng,Qianyun Nie,Hao Yu,Wenjuan Dong,Bingzheng Chen,Chunhui Song,Yan Tian,Lixiang Qin,Junjie Shu,Shijun Hong,Lihua Li
View all publications
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Product promise

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