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AB254707

Anti-MECR antibody

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(2 Publications)

Rabbit Polyclonal MECR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MECR aa 250 to C-terminus.

View Alternative Names

NBRF1, CGI-63, MECR, 2-enoyl thioester reductase, Nuclear receptor-binding factor 1, HsNrbf-1, NRBF-1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Paraffin-embedded human cerebral cortex tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MECR antibody (AB254707)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MECR antibody (AB254707)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling MECR using ab254707 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Paraffin-embedded human kidney tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-MECR antibody (AB254707)
  • WB

Supplier Data

Western blot - Anti-MECR antibody (AB254707)

All lanes:

Western blot - Anti-MECR antibody (ab254707) at 0.4 µg/mL

Lane 1:

RT4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 40 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Paraffin-embedded human colon tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Paraffin-embedded human testis tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-MECR antibody (AB254707)
  • WB

Supplier Data

Western blot - Anti-MECR antibody (AB254707)

All lanes:

Western blot - Anti-MECR antibody (ab254707) at 0.4 µg/mL

Lane 1:

NIH/3T3 cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MECR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BV79

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MECR or mitochondrial trans-2-enoyl-CoA reductase plays an important role in mitochondrial fatty acid synthesis. This enzyme is involved in the final reduction step of trans-2-enoyl-CoA to acyl-CoA. Known alternately as NRBF1 MECR has a molecular mass of approximately 37 kDa. It finds expression primarily in mitochondria-rich tissues like heart liver and skeletal muscle where energy production and fatty acid metabolism are highly active.
Biological function summary

The MECR protein contributes to the biosynthesis of mitochondrial fatty acids by catalyzing the reduction of trans-2-enoyl-CoA. It operates as part of a multicomponent enzyme complex involved in mitochondrial fatty acid synthesis. This process is necessary for maintaining proper mitochondrial membrane integrity and function affecting multiple cellular functions beyond lipid metabolism including regulation of energy homeostasis.

Pathways

MECR integrates mainly into mitochondrial fatty acid synthesis pathways. It collaborates with other enzymes to regulate fatty acid metabolism contributing to energy production within mitochondria. MECR partners with the protein ETFDH which transfers electrons necessary for the enzymatic reactions within the pathway. Further it interacts indirectly with the beta-oxidation pathway which breaks down fatty acids into acyl-CoA units subsequently entering the mitochondrial electron transport chain for ATP production.

Defective MECR activity has been linked to progressive neurological disorders and cardiomyopathy. Disorders like mitochondrial myopathy are associated with mutations affecting MECR's enzymatic efficiency. Other proteins such as ACAD9 connect with MECR where both participate in similar or adjacent pathways disrupted in these disorders. This link emphasizes the potential role of MECR in therapeutic strategies targeting mitochondrial dysfunction-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (PubMed : 12654921, PubMed : 18479707, PubMed : 27817865). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity). Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism (PubMed : 37653044). Required for proper morphology and bioenergetic functions of mitochondria (PubMed : 37653044). Required for maintenance of neurons (By similarity).
See full target information MECR
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39859268

2025

Identification of Downregulated Gene in Parkinson's Disease Through Integrated Transcriptomic Analysis and Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Danlei Wang,Haoheng Yu,Yi Qu,Ke An,Hongming Liang,Zhijuan Mao,Jingyi Li,Yongjie Xiong,Zhe Min,Zheng Xue

Aging cell 23:e14320 PubMed39158018

2024

Single-cell RNA sequencing reveals the CRTAC1 population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1 macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Tang,Dachun Zhuo,Yuexin Yu,Weilin Pu,Yanyun Ma,Yuting Zhang,Yan Huang,Qing Zhang,Kunhai Tang,Chen Meng,Di Yang,Lu Bai,Dongyi He,Li Jin,Hejian Zou,Huji Xu,Qi Zhu,Jiucun Wang,Yuanyuan Chen,Jing Liu
View all publications
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