Rabbit Polyclonal MECR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human MECR aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MECR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BV79
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (PubMed:12654921, PubMed:18479707, PubMed:27817865). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity). Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism (PubMed:37653044). Required for proper morphology and bioenergetic functions of mitochondria (PubMed:37653044). Required for maintenance of neurons (By similarity).
Alternative names
NBRF1, CGI-63, MECR, 2-enoyl thioester reductase, Nuclear receptor-binding factor 1, HsNrbf-1, NRBF-1
Recommended products
Rabbit Polyclonal MECR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human MECR aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MECR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BV79
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MECR or mitochondrial trans-2-enoyl-CoA reductase plays an important role in mitochondrial fatty acid synthesis. This enzyme is involved in the final reduction step of trans-2-enoyl-CoA to acyl-CoA. Known alternately as NRBF1 MECR has a molecular mass of approximately 37 kDa. It finds expression primarily in mitochondria-rich tissues like heart liver and skeletal muscle where energy production and fatty acid metabolism are highly active.
- Biological function summary
The MECR protein contributes to the biosynthesis of mitochondrial fatty acids by catalyzing the reduction of trans-2-enoyl-CoA. It operates as part of a multicomponent enzyme complex involved in mitochondrial fatty acid synthesis. This process is necessary for maintaining proper mitochondrial membrane integrity and function affecting multiple cellular functions beyond lipid metabolism including regulation of energy homeostasis.
- Pathways
MECR integrates mainly into mitochondrial fatty acid synthesis pathways. It collaborates with other enzymes to regulate fatty acid metabolism contributing to energy production within mitochondria. MECR partners with the protein ETFDH which transfers electrons necessary for the enzymatic reactions within the pathway. Further it interacts indirectly with the beta-oxidation pathway which breaks down fatty acids into acyl-CoA units subsequently entering the mitochondrial electron transport chain for ATP production.
- Associated diseases and disorders
Defective MECR activity has been linked to progressive neurological disorders and cardiomyopathy. Disorders like mitochondrial myopathy are associated with mutations affecting MECR's enzymatic efficiency. Other proteins such as ACAD9 connect with MECR where both participate in similar or adjacent pathways disrupted in these disorders. This link emphasizes the potential role of MECR in therapeutic strategies targeting mitochondrial dysfunction-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (ab254707)
Paraffin-embedded human testis tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-MECR antibody (ab254707)
PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling MECR using ab254707 at 4µg/ml (green) in ICC/IF.
Western blot - Anti-MECR antibody (ab254707)
All lanes: Western blot - Anti-MECR antibody (ab254707) at 0.4 µg/mL
Lane 1: RT4 cell lysate
Lane 2: U-251 MG cell lysate
Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (ab254707)
Paraffin-embedded human colon tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (ab254707)
Paraffin-embedded human kidney tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (ab254707)
Paraffin-embedded human cerebral cortex tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.
Western blot - Anti-MECR antibody (ab254707)
All lanes: Western blot - Anti-MECR antibody (ab254707) at 0.4 µg/mL
Lane 1: NIH/3T3 cell lysate
Lane 2: NBT-II cell lysate
Predicted band size: 40 kDa
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