AB327176

Anti-MED12 Antibody

Name: Anti-MED12 Antibody (ab327176) | Abcam
Brand: Abcam Limited
SKU: AB327176
Price: 560 USD
Availability: InStock

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Rabbit Polyclonal MED12 antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MED12.

View Alternative Names

ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230, MED12, Mediator of RNA polymerase II transcription subunit 12, Activator-recruited cofactor 240 kDa component, CAG repeat protein 45, Mediator complex subunit 12, OPA-containing protein, Thyroid hormone receptor-associated protein complex 230 kDa component, Trinucleotide repeat-containing gene 11 protein, Trap230

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human placenta using ab327176 at 1 : 20–1 : 50 dilution, showing high expression.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human cerebral cortex, colon, spleen and testis using ab327176 at 1 : 20–1 : 50 dilution (A) shows similar protein distribution across tissues to an independent Anti-MED12 antibody (B).

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human spleen using ab327176 at 1 : 20–1 : 50 dilution.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemistry analysis in human placenta and pancreas tissues using ab327176 at 1 : 20–1 : 50 dilution. Corresponding MED12 RNA-seq data are presented for the same tissues.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human cerebral cortex using ab327176 at 1 : 20–1 : 50 dilution.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human colon using ab327176 at 1 : 20–1 : 50 dilution.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human pancreas using ab327176 at 1 : 20–1 : 50 dilution, showing low expression as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human testis using ab327176 at 1 : 20–1 : 50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MED12 Antibody (AB327176)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MED12 Antibody (AB327176)

Immunofluorescent staining of human cell line U-2 OS using ab327176 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP

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ChIP - Anti-MED12 Antibody (AB327176)

ChIP-Exo-Seq composite graph for ab327176 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Supplier Data

Western blot - Anti-MED12 Antibody (AB327176)

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using ab327176 at 0.04–0.4 µg/ml. Remaining relative intensity is presented.

All lanes:

Western blot - Anti-MED12 Antibody (ab327176)

Lane 2:

U2OS cells transfected with control siRNA

Lane 3:

U2OS cells transfected with target specific siRNA probe #1

Lane 4:

U2OS cells transfected with target specific siRNA probe #2

Predicted band size: 243.08 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP, WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MED12.

Q93074

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.

See full target information MED12

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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