AB70842

Anti-MED12 antibody

Name: Anti-MED12 antibody (ab70842) | Abcam
Brand: Abcam Limited
SKU: AB70842
Price: 435 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(14 Publications)

Rabbit Polyclonal MED12 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human MED12 aa 2150-2200.

View Alternative Names

ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230, MED12, Mediator of RNA polymerase II transcription subunit 12, Activator-recruited cofactor 240 kDa component, CAG repeat protein 45, Mediator complex subunit 12, OPA-containing protein, Thyroid hormone receptor-associated protein complex 230 kDa component, Trinucleotide repeat-containing gene 11 protein, Trap230

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody (AB70842)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody (AB70842)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED12 with ab70842 at 1/1000 (1µg/ml). Detection : DAB.

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Immunoprecipitation - Anti-MED12 antibody (AB70842)

Detection of Human MED12 by Immunoprecipitation using Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70842 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using ab70842 at 1 µg/ml.

All lanes:

Immunoprecipitation - Anti-MED12 antibody (ab70842)

Predicted band size: 243 kDa

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Western blot - Anti-MED12 antibody (AB70842)

All lanes:

Western blot - Anti-MED12 antibody (ab70842) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Lane 5:

Whole cell lysate from NIH 3T3 cells at 50 µg

Predicted band size: 243 kDa

Observed band size: 238 kDa,65 kDa

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Exposure time: 3min

CiteAb

Western blot - Anti-MED12 antibody (AB70842)

MED12 western blot using anti-MED12 antibody ab70842. Publication image and figure legend from Shishkova, E., Zeng, H., et al., 2017, Nat Commun, PubMed 28537268.

ab70842 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70842 please see the product overview.

Requirement of the N-terminal domain for substrate recognition by CARM1.(a) Schematic diagram of FL and N-terminal truncated CARM1 derivatives. CARM1 FL protein contained 608 residues. CARM1 28–608 lacks the first, unstructured 28 residues denoted in grey. CARM1 140–608 lacks the first 140 residues encompassing the EVH1 domain denoted in green. (b) Western blot analyses of co-immunoprecipitated BAF155, MED12, PABP1, NCOA3 and TET2 with FLAG-tagged CARM1, transiently transfected into HEK293T CARM1 KO cells. CARM1 was immunoprecipiated with the anti-FLAG antibody, and the presence of BAF155, MED12, PABP1, NCOA3 and TET2 in the immunoprecipitates was detected with western blots using the respective antibodies. The loading controls are depicted below the corresponding western blot results, separately for BAF155, MED12 and PABP1, and the other two proteins. In all cases the amount of co-precipitated protein was strongly reduced in cell lines expressing N-terminus truncated CARM1 140–608. (c) Western blot analyses of total ADMA-containing proteins co-precipitated with CARM1. The FLAG-tagged CARM1 immunoprecipitates in b were probed with ADMA antibodies. The strong reduction in the levels of ADMA—containing proteins in cells expressing CARM1 140–680 was evident on both short (left) and long exposure (right). The corresponding loading control was shared between the experiments in b (BAF155, MED12 and PABP1) and c and is depicted in b labelled with IB : FLAG. (d) FP assay using purified recombinant 6xHis-CARM1 28–140 and fluorescein-labelled BAF155 peptide. Pronounced increase in FP was observed at high concentrations of recombinant CARM1, but not with the BSA control, demonstrating that the EVH1 domain of CARM1 directly interacts with the enzyme's substrate at low affinity. (e) Coomassie Blue staining of highly purified recombinant 6xHis-CARM1 28–140 used in the FP assay (d).

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human MED12 aa 2150-2200. The exact immunogen used to generate this antibody is proprietary information.

Q93074

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED12 also known as mediator complex subunit 12 is a component of the transcriptional Mediator complex. It weighs approximately 250 kDa and is widely expressed in many tissues showing high transcriptional regulatory activity. MED12 collaborates with other subunits within the Mediator complex to transmit signals from regulatory DNA elements to RNA polymerase II facilitating gene expression regulation.

Biological function summary

MED12 functions as a core component of the larger Mediator complex which plays an important role in controlling RNA polymerase II-dependent genes. Through interactions with activators and repressors MED12 participates in chromatin modification and looping affecting gene transcription rates. It is enriched in tissues where fine control of gene expression is essential impacting growth differentiation and development processes.

Pathways

MED12 integrates into critical signaling pathways including the Wnt/β-catenin and Hedgehog pathways. In these pathways MED12 interacts with other proteins like β-catenin serving as a connecting node that influences the transcription of target genes essential for cell proliferation and differentiation. Such interactions help coordinate cellular responses to external signals and ensure proper pathway regulation.

MED12 mutations have been linked to disorders like Lujan-Fryns syndrome and uterine leiomyomas. In these contexts MED12 interacts with G protein-coupled receptors and other transcription factors such as β-catenin resulting in dysregulation that leads to the development of these conditions. Understanding the connections between MED12 and associated proteins provides insights into the underlying mechanisms of these pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.

See full target information MED12

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1180 PubMed40775066

2025

Regulation of cortical neurogenesis by MED13L via transcriptional priming and its implications for MED13L syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Li,Yu-Zhu Hao,Jia Cui,Xiao-Xiao Cao,Nan Wu,Ji Ma,Shuang-Yi Xie,Yi-Hsuan Pan,Xue-Lian He,Yu-Lan Zhao,Xiao-Bing Yuan

American journal of cancer research 13:1999-2012 PubMed37293147

2023

Exosomal transfer of miR-548aq-3p confers cisplatin resistance MED12 downregulation in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlong Shi,Yuwei Zou,Yu Guo,Yulin Liu,Qiang Wang

Cell reports 41:111852 PubMed36543134

2022

MED13 and glycolysis are conserved modifiers of α-synuclein-associated neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mengda Ren,Ying Yang,Kelsey Hwee Yee Heng,Lu Yi Ng,Claris Yuin-Yi Chong,Yan Ting Ng,Srinivas Gorur-Shandilya,Rachel Min Qi Lee,Kah Leong Lim,Jing Zhang,Tong-Wey Koh

Molecular metabolism 48:101227 PubMed33812059

2021

The Mediator complex kinase module is necessary for fructose regulation of liver glycogen levels through induction of glucose-6-phosphatase catalytic subunit (G6pc).

Applications

Unspecified application

Species

Unspecified reactive species

Dou Yeon Youn,Alus M Xiaoli,Haihong Zong,Junichi Okada,Li Liu,Jacob Pessin,Jeffrey E Pessin,Fajun Yang

Cell death & disease 10:387 PubMed31097718

2019

Smad7:β-catenin complex regulates myogenic gene transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Soma Tripathi,Tetsuaki Miyake,John C McDermott

Molecular cancer 18:93 PubMed31072327

2019

MED12 exerts an emerging role in actin-mediated cytokinesis via LIMK2/cofilin pathway in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Xu,Fang Wang,Guibo Li,Xiaokun Wang,Xiaona Fang,Haoxuan Jin,Zhen Chen,Jianye Zhang,Liwu Fu

The Journal of biological chemistry 294:9076-9083 PubMed31028171

2019

The subunit assembly state of the Mediator complex is nutrient-regulated and is dysregulated in a genetic model of insulin resistance and obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Dou Yeon Youn,Alus M Xiaoli,Hyokjoon Kwon,Fajun Yang,Jeffrey E Pessin

Molecular cell 73:250-263.e5 PubMed30527662

2018

Promoter-Enhancer Communication Occurs Primarily within Insulated Neighborhoods.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Sun,Constantinos Chronis,Michael Kronenberg,Xiao-Fen Chen,Trent Su,Fides D Lay,Kathrin Plath,Siavash K Kurdistani,Michael F Carey

eNeuro 4: PubMed28955722

2017

The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent.

Applications

Unspecified application

Species

Unspecified reactive species

Kalpana D Acharya,Sabin A Nettles,Katherine J Sellers,Dana D Im,Moriah Harling,Cassandra Pattanayak,Didem Vardar-Ulu,Cheryl F Lichti,Shixia Huang,Dean P Edwards,Deepak P Srivastava,Larry Denner,Marc J Tetel

Nature communications 8:15571 PubMed28537268

2017

Global mapping of CARM1 substrates defines enzyme specificity and substrate recognition.

Applications

Species

Unspecified reactive species

Evgenia Shishkova,Hao Zeng,Fabao Liu,Nicholas W Kwiecien,Alexander S Hebert,Joshua J Coon,Wei Xu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MED12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody (AB70842)

Immunoprecipitation - Anti-MED12 antibody (AB70842)

Western blot - Anti-MED12 antibody (AB70842)

Western blot - Anti-MED12 antibody (AB70842)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise