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AB300154

Anti-MED12 antibody [EPR25211-36]

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Rabbit Recombinant Monoclonal MED12 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples.

View Alternative Names

ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230, MED12, Mediator of RNA polymerase II transcription subunit 12, Activator-recruited cofactor 240 kDa component, CAG repeat protein 45, Mediator complex subunit 12, OPA-containing protein, Thyroid hormone receptor-associated protein complex 230 kDa component, Trinucleotide repeat-containing gene 11 protein, Trap230

1 Images
Western blot - Anti-MED12 antibody [EPR25211-36] (AB300154)
  • WB

Lab

Western blot - Anti-MED12 antibody [EPR25211-36] (AB300154)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1 : 59 seconds; Lanes 2-6 : 15 seconds

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28183795; PMID : 29440396 ).

The lower bands may be caused by degradation.

All lanes:

Western blot - Anti-MED12 antibody [EPR25211-36] (ab300154) at 1/1000 dilution

Lane 1:

Mouse uterus tissue lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 240 kDa

false

  • Carrier free

    Anti-MED12 antibody [EPR25211-36] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25211-36

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED12 also known as mediator complex subunit 12 is a component of the transcriptional Mediator complex. It weighs approximately 250 kDa and is widely expressed in many tissues showing high transcriptional regulatory activity. MED12 collaborates with other subunits within the Mediator complex to transmit signals from regulatory DNA elements to RNA polymerase II facilitating gene expression regulation.
Biological function summary

MED12 functions as a core component of the larger Mediator complex which plays an important role in controlling RNA polymerase II-dependent genes. Through interactions with activators and repressors MED12 participates in chromatin modification and looping affecting gene transcription rates. It is enriched in tissues where fine control of gene expression is essential impacting growth differentiation and development processes.

Pathways

MED12 integrates into critical signaling pathways including the Wnt/β-catenin and Hedgehog pathways. In these pathways MED12 interacts with other proteins like β-catenin serving as a connecting node that influences the transcription of target genes essential for cell proliferation and differentiation. Such interactions help coordinate cellular responses to external signals and ensure proper pathway regulation.

MED12 mutations have been linked to disorders like Lujan-Fryns syndrome and uterine leiomyomas. In these contexts MED12 interacts with G protein-coupled receptors and other transcription factors such as β-catenin resulting in dysregulation that leads to the development of these conditions. Understanding the connections between MED12 and associated proteins provides insights into the underlying mechanisms of these pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
See full target information MED12
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Product promise

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