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AB229717

Anti-MED13/GCT antibody

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(1 Publication)

Rabbit Polyclonal MED13/GCT antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MED13.

View Alternative Names

ARC250, KIAA0593, THRAP1, TRAP240, MED13, Mediator of RNA polymerase II transcription subunit 13, Activator-recruited cofactor 250 kDa component, Mediator complex subunit 13, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, Vitamin D3 receptor-interacting protein complex component DRIP250, Trap240, DRIP250

1 Images
Western blot - Anti-MED13/GCT antibody (AB229717)
  • WB

Supplier Data

Western blot - Anti-MED13/GCT antibody (AB229717)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-MED13/GCT antibody (ab229717) at 1/500 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 239 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human MED13. The exact immunogen used to generate this antibody is proprietary information.

Q9UHV7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED13 also known as Mediator complex subunit 13 is a significant protein component of the Mediator complex which is important for transcription regulation by RNA polymerase II. This protein with a mass around 200 kDa is widely expressed in various tissues including the liver heart and kidney. MED13 interacts with multiple transcriptional activators and is integral to the assembly and stability of the Mediator complex influencing gene expression.
Biological function summary

Mediator complex subunit 13 acts as a gatekeeper in gene expression regulation by integrating signals from transcription factors to initiate transcription by RNA polymerase II. MED13 is an essential part of the CDK8 submodule within the Mediator complex impacting its regulatory role in transcription activation and repression. Its function tightly connects to the control of cell growth development and metabolism impacting how genes are expressed in response to cellular signals.

Pathways

MED13 plays an important role in the Wnt/β-catenin and nuclear receptor signaling pathways. These pathways impact the regulation of development cell proliferation and metabolic processes. MED13 coordinates with proteins like CDK8 visiting these pathways to influence transcriptional outcomes. Through these coordinated actions MED13 helps regulate signals that guide cellular behavior and differentiation.

Disruptions in the function of MED13 can contribute to various conditions. Abnormal MED13 activity has been linked to heart diseases specifically cardiac hypertrophy and metabolic disorders like obesity. In the context of these diseases MED13 interacts with other proteins like GATA4 and PPARγ which play roles in the pathological processes underlying these conditions. Understanding MED13's involvement in these disorders offers important insights into potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 23:6822-6834 PubMed31386303

2019

Molecular mechanism of tumour necrosis factor alpha regulates hypocretin (orexin) expression, sleep and behaviour.

Applications

Unspecified application

Species

Unspecified reactive species

Shuqin Zhan,Pulin Che,Xue-Ke Zhao,Ning Li,Yan Ding,Jianghong Liu,Spring Li,Karyn Ding,Lynn Han,Zhaoyang Huang,Liyong Wu,Yuping Wang,Meng Hu,Xiaosi Han,Qiang Ding
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Product promise

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For full details, please see our Terms & Conditions

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