AB76923

Anti-MED13/GCT antibody

Name: Anti-MED13/GCT antibody (ab76923) | Abcam
Brand: Abcam Limited
SKU: AB76923
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal MED13/GCT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MED13 aa 300-400.

View Alternative Names

ARC250, KIAA0593, THRAP1, TRAP240, MED13, Mediator of RNA polymerase II transcription subunit 13, Activator-recruited cofactor 250 kDa component, Mediator complex subunit 13, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, Vitamin D3 receptor-interacting protein complex component DRIP250, Trap240, DRIP250

2 Images

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Immunoprecipitation - Anti-MED13/GCT antibody (AB76923)

Detection of MED13/GCT by Western Blot of Immunprecipitate.
ab76923 at 1μg/ml staining MED13/GCT in HeLa whole cell lysate immunoprecipitated using ab76923 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-MED13/GCT antibody (ab76923)

Predicted band size: 239 kDa

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Unknown

Western blot - Anti-MED13/GCT antibody (AB76923)

All lanes:

Western blot - Anti-MED13/GCT antibody (ab76923) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 239 kDa

Observed band size: 300 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human MED13 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q9UHV7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED13 also known as Mediator complex subunit 13 is a significant protein component of the Mediator complex which is important for transcription regulation by RNA polymerase II. This protein with a mass around 200 kDa is widely expressed in various tissues including the liver heart and kidney. MED13 interacts with multiple transcriptional activators and is integral to the assembly and stability of the Mediator complex influencing gene expression.

Biological function summary

Mediator complex subunit 13 acts as a gatekeeper in gene expression regulation by integrating signals from transcription factors to initiate transcription by RNA polymerase II. MED13 is an essential part of the CDK8 submodule within the Mediator complex impacting its regulatory role in transcription activation and repression. Its function tightly connects to the control of cell growth development and metabolism impacting how genes are expressed in response to cellular signals.

Pathways

MED13 plays an important role in the Wnt/β-catenin and nuclear receptor signaling pathways. These pathways impact the regulation of development cell proliferation and metabolic processes. MED13 coordinates with proteins like CDK8 visiting these pathways to influence transcriptional outcomes. Through these coordinated actions MED13 helps regulate signals that guide cellular behavior and differentiation.

Disruptions in the function of MED13 can contribute to various conditions. Abnormal MED13 activity has been linked to heart diseases specifically cardiac hypertrophy and metabolic disorders like obesity. In the context of these diseases MED13 interacts with other proteins like GATA4 and PPARγ which play roles in the pathological processes underlying these conditions. Understanding MED13's involvement in these disorders offers important insights into potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

See full target information MED13

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 10:387 PubMed31097718

2019

Smad7:β-catenin complex regulates myogenic gene transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Soma Tripathi,Tetsuaki Miyake,John C McDermott

Human genetics 137:375-388 PubMed29740699

2018

De novo mutations in MED13, a component of the Mediator complex, are associated with a novel neurodevelopmental disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Lot Snijders Blok,Susan M Hiatt,Kevin M Bowling,Jeremy W Prokop,Krysta L Engel,J Nicholas Cochran,E Martina Bebin,Emilia K Bijlsma,Claudia A L Ruivenkamp,Paulien Terhal,Marleen E H Simon,Rosemarie Smith,Jane A Hurst,Heather McLaughlin,Richard Person,Amy Crunk,Michael F Wangler,Haley Streff,Joseph D Symonds,Sameer M Zuberi,Katherine S Elliott,Victoria R Sanders,Abigail Masunga,Robert J Hopkin,Holly A Dubbs,Xilma R Ortiz-Gonzalez,Rolph Pfundt,Han G Brunner,Simon E Fisher,Tjitske Kleefstra,Gregory M Cooper

PloS one 8:e72496 PubMed23967313

2013

Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene.

Applications

Unspecified application

Species

Unspecified reactive species

Aya Fukuda,Miho Shimada,Tomoyoshi Nakadai,Ken Nishimura,Koji Hisatake

Journal of virology 87:3425-34 PubMed23302885

2013

Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex.

Applications

Unspecified application

Species

Human

S Vijayalingam,G Chinnadurai

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MED13/GCT antibody

Immunoprecipitation - Anti-MED13/GCT antibody (AB76923)

Western blot - Anti-MED13/GCT antibody (AB76923)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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