Rabbit Polyclonal MED13L antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MED13L aa 550-600.
View Alternative Names
KIAA1025, PROSIT240, THRAP2, TRAP240L, MED13L, Mediator of RNA polymerase II transcription subunit 13-like, Mediator complex subunit 13-like, Thyroid hormone receptor-associated protein 2, Thyroid hormone receptor-associated protein complex 240 kDa component-like
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED13L antibody (AB87831)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED13L with ab87831 at 1/1000 (1µg/ml). Detection : DAB.
- IP
Unknown
Immunoprecipitation - Anti-MED13L antibody (AB87831)
Detection of MED13L by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87831 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection. Chemiluminescence with an exposure time of 30 seconds.
All lanes:
Immunoprecipitation - Anti-MED13L antibody (ab87831) at 1 µg/mL
Lane 1:
For WB: HeLa whole cell lysate at 50ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Lane 2:
For WB: HeLa whole cell lysate at 15ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Lane 3:
For WB: HeLa whole cell lysate at 5ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Predicted band size: 243 kDa
true
Exposure time: 30s
- WB
Unknown
Western blot - Anti-MED13L antibody (AB87831)
All lanes:
Western blot - Anti-MED13L antibody (ab87831) at 0.1 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg/mL
Lane 2:
HeLa whole cell lysate at 15 µg/mL
Lane 3:
HeLa whole cell lysate at 5 µg/mL
Predicted band size: 243 kDa
true
Exposure time: 3min
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The MED13L protein is integral to the regulation of gene transcription. It functions within the larger context of the Mediator complex which serves as a bridge linking gene-specific transcription factors to the RNA polymerase II enzyme. By modulating the interaction between these transcription factors and polymerase MED13L helps control the expression of target genes. This regulation is important for the proper development and maintenance of many cellular processes particularly in nervous tissues.
Pathways
MED13L is involved in significant biological processes such as cell differentiation and growth. It participates in the Wnt signaling pathway which plays a critical role in embryonic development and cancer pathogenesis. Within this pathway MED13L interacts with other proteins like β-catenin to promote transcriptional activation necessary for cell growth and differentiation. Additionally its role links it to the regulation of the Hedgehog signaling pathway another essential pathway for developmental processes.
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Target data
Publications (2)
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Human mutation 35:1311-20 PubMed25137640
2014
Applications
Unspecified application
Species
Unspecified reactive species
Nature structural & molecular biology 20:611-9 PubMed23563140
2013
Applications
WB
Species
Human
Complementary Products
Product promise
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