AB221741

Anti-MED25 antibody

Name: Anti-MED25 antibody (ab221741) | Abcam
Brand: Abcam Limited
SKU: AB221741
Price: 645 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MED25 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MED25 aa 600-700.

View Alternative Names

ACID1, ARC92, PTOV2, TCBAP0758, MED25, Mediator of RNA polymerase II transcription subunit 25, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, Mediator complex subunit 25, p78

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MED25 antibody (AB221741)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MED25 antibody (AB221741)

PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for MED25 (green) using ab221741 at 4 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-MED25 antibody (AB221741)

All lanes:

Western blot - Anti-MED25 antibody (ab221741) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 (human brain glioma cell line) cell lysate

Predicted band size: 78 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MED25 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

Q71SY5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED25 also known as ARC92 is a subunit of the mediator complex a multiprotein structure that modulates transcription of RNA polymerase II-dependent genes. MED25 has a molecular mass of approximately 80 kDa and shows expression in various tissues including the brain liver and heart. The protein serves as an important link between transcription factors and the basal transcription machinery functioning mechanically as a scaffold facilitating the assembly of transcriptional complexes.

Biological function summary

The MED25 protein plays an essential role in regulating gene expression by interacting with transcription factors. MED25 is part of the larger mediator complex which acts as a bridge to convey regulatory signals from enhancers to promoters. This interaction influences development processes and cellular differentiation by controlling the transcription of specific genes necessary for these functions.

Pathways

MED25 is integral to the transcriptional regulation within the Wnt signaling and TGF-beta pathways. It mediates interactions between transcription factors such as STAT3 and p53 and the transcriptional machinery facilitating signal transduction leading to gene expression changes. These pathways regulate several biological processes including cell growth and apoptotic responses establishing MED25 as an important player in maintaining cellular homeostasis.

MED25 has been implicated in neurological disorders such as Charcot-Marie-Tooth disease. MED25 mutations have been associated with this peripheral neuropathy affecting nerve conduction. Additionally disruptions in MED25 interactions with the transcription machinery have connections to cancer development since its role in pathways like TGF-beta involves proteins like SMAD3 that regulate cell proliferation and apoptosis. Understanding MED25's function and interactions offers potential therapeutic avenues for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.

See full target information MED25

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 14:6815-6822 PubMed29422958

2017

Expression and function of tetraspanin 1 in esophageal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Gu,Weiwei Chen,Li Chen,Guilan Wang,Tiejun Li,Yuanyuan Zhu,Xiaojiao Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MED25 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MED25 antibody (AB221741)

Western blot - Anti-MED25 antibody (AB221741)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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