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AB73294

Anti-MED28/EG1 antibody

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(1 Publication)

Rabbit Polyclonal MED28/EG1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EG1, FKSG20, MED28, Mediator of RNA polymerase II transcription subunit 28, Endothelial-derived protein 1, Mediator complex subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Tumor angiogenesis marker EG-1, Magicin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED28/EG1 antibody (AB73294)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED28/EG1 antibody (AB73294)

ab73294 at 2.5μg/ml staining MED28/EG1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

Western blot - Anti-MED28/EG1 antibody (AB73294)
  • WB

Unknown

Western blot - Anti-MED28/EG1 antibody (AB73294)

All lanes:

Western blot - Anti-MED28/EG1 antibody (ab73294) at 1 µg/mL

Lane 1:

human brain tissue lysate at 15 µg

Lane 2:

human brain tissue lysate at 15 µg with blocking peptide

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MED28 also known as EG1 is an integral component of the mediator complex which plays an important role in regulating transcription. MED28 is a protein with a molecular mass of approximately 28 kDa. It is expressed in various tissues with notable presence in cardiac and skeletal muscle. The expression pattern suggests its involvement in complex cellular processes across different tissues.
Biological function summary

The MED28/EG1 protein serves as part of the larger mediator complex which connects transcription factors to RNA polymerase II during the transcription cycle. This complex acts as a transcriptional coactivator essential for the transcription of almost all class II gene promoters. MED28's contribution involves regulation of various genes hence it has a significant role in development and differentiation processes.

Pathways

MED28/EG1 is involved in essential pathways including the Wnt signaling pathway. It participates in modulating gene expression through interactions with other mediator complex proteins contributing to transcriptional regulation important for cellular responses and signaling networks. Other related proteins such as MED8 and MED12 alongside MED28 offer a cooperative function in mediator complex activities.

Aberrations in the function of MED28/EG1 associate with several conditions such as cancer and cardiovascular diseases. Dysregulation in its expression or mediator complex interactions could lead to inconsistencies in transcriptional activation contributing to the progression of these diseases. Studies have linked MED28 to proteins such as cyclin-dependent kinases which influence disease states by controlling cell cycle regulation and proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
See full target information MED28
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 21:87-95 PubMed35785044

2022

NUP160 knockdown inhibits the progression of diabetic nephropathy and .

Applications

Unspecified application

Species

Unspecified reactive species

Jiayong Xie,Zhi Chen,Gang Yao,Ying Yuan,Wenjuan Yu,Qiang Zhu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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