JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB192309

Anti-MED29/med2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal MED29/med2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MED29.

View Alternative Names

IXL, MED29, Mediator of RNA polymerase II transcription subunit 29, Intersex-like protein, Mediator complex subunit 29

1 Images
Western blot - Anti-MED29/med2 antibody (AB192309)
  • WB

Supplier Data

Western blot - Anti-MED29/med2 antibody (AB192309)

All lanes:

Western blot - Anti-MED29/med2 antibody (ab192309)

Lane 1:

HEK293T whole cell lysate

Lane 2:

Mouse brain tissue lysate

Lane 3:

Rat lung tissue lysate

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MED29. The exact immunogen used to generate this antibody is proprietary information.

Q9NX70

style
left-column

Complementary Products

Primary Antibodies

AB190936

Anti-SSR3 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-SSR3 antibody - N-terminal (AB190936)

  • 0
  • 0
Primary Antibodies

AB191691

Anti-UBIAD1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBIAD1 antibody - C-terminal (AB191691)

  • 0
  • 0
Primary Antibodies

AB192438

Anti-SLC28A1 antibody

Western blot - Anti-SLC28A1 antibody (AB192438)

  • 0
  • 0
Primary Antibodies

AB191143

Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (AB191143)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purity > 95% (by SDS-PAGE)
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED29 also known as Med2 is a component of the Mediator complex a multi-subunit assembly essential for the transcription regulation of RNA polymerase II. MED29 has an approximate mass of 29 kDa. It is expressed in various human tissues with high levels noted in brain heart and kidney tissues. The protein functions by acting as a coactivator modulating signals between transcriptional activators and the RNA polymerase II machinery. This allows MED29 to play a significant role in the control of gene expression.
Biological function summary

MED29 contributes to cellular processes through its role in the Mediator complex. This complex serves as a bridge facilitating communication between gene-specific transcription factors and the polymerase core machinery influencing transcription initiation. MED29 impacts the regulation of genes linked to cell growth development and stress responses. Given its participation in these important processes MED29 ensures that transcription responds accurately to changes in the cellular environment.

Pathways

MED29 plays a significant role in transcription regulatory pathways particularly the RNA polymerase II transcription pathway. MED29 interacts closely with other Mediator complex proteins such as MED1 and MED14 to drive precise gene transcription. These interactions facilitate a well-coordinated transcriptional response required for normal cellular functioning and response to stimuli.

MED29 has implications in cancer and cardiovascular disorders. Aberrant expression of MED29 links to its role in tumorigenesis supporting abnormal cell proliferation and survival. Similarly variations in MED29 expression impact heart function contributing to the development of cardiovascular diseases. MED29's association with other Mediator components such as MED12 further highlights its involvement in these conditions signifying its potential as a therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED29
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 10:11 PubMed38291322

2024

Structural insights into the specific recognition of mitochondrial ribosome-binding factor hsRBFA and 12 S rRNA by methyltransferase METTL15.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Lv,Wanwan Zhou,Yijie Hao,Fudong Li,Huafeng Zhang,Xuebiao Yao,Yunyu Shi,Liang Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com