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AB192523

Anti-MED30 / TRAP25 antibody

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(1 Publication)

Rabbit Polyclonal MED30 / TRAP25 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MED30.

View Alternative Names

THRAP6, TRAP25, MED30, Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30, TRAP/Mediator complex component TRAP25, Thyroid hormone receptor-associated protein 6, Thyroid hormone receptor-associated protein complex 25 kDa component, Trap25

1 Images
Western blot - Anti-MED30 / TRAP25 antibody (AB192523)
  • WB

Supplier Data

Western blot - Anti-MED30 / TRAP25 antibody (AB192523)

All lanes:

Western blot - Anti-MED30 / TRAP25 antibody (ab192523)

Lane 1:

HEK293T whole cell lysate

Lane 2:

Raw264.7 whole cell lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MED30. The exact immunogen used to generate this antibody is proprietary information.

Q96HR3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purity is > 95% (by SDS-PAGE).
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED30 also known as TRAP25 is a component of the Mediator complex which functions as a coactivator in transcription. It possesses a molecular mass of approximately 21 kDa. This protein is expressed in a broad range of tissues indicating its ubiquitous role across different biological systems. MED30 forms part of the complex machinery that regulates transcription by serving as a bridge between gene-specific transcription factors and RNA polymerase II.
Biological function summary

MED30 participates in essential processes by integrating signals that are required for the initiation and regulation of transcription. It is a part of the larger Mediator complex which is important for the transcription of nearly all protein-coding genes. The Mediator complex consists of several subunits and MED30 helps in maintaining the structural stability and functional modulation of this assembly contributing to transcription regulation at various levels.

Pathways

MED30 integrates signal transduction pathways with transcriptional regulation. It plays a significant role within the MAPK/ERK and Wnt signaling pathways. In the MAPK/ERK pathway MED30 interacts with key proteins such as ERK1/2 to mediate responses to external stimuli affecting gene expression. Regarding the Wnt pathway it collaborates with beta-catenin linking signaling alterations to changes in transcriptional activity.

MED30 has shown associations with certain cancers and developmental disorders. Abnormalities in the Mediator complex including MED30 have been linked to oncogenesis where irregular expression or mutations could lead to tumor progression. Additionally the dysregulation of Mediator subunits including MED30 connects to developmental syndromes where altered gene expression patterns impair normal developmental processes. Connections between MED30 and other proteins such as beta-catenin in Wnt-related cancers highlight its importance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED30
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0270880 PubMed35857794

2022

ATF4 promotes brain vascular smooth muscle cells proliferation, invasion and migration by targeting miR-552-SKI axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meina Feng,Qin Zhou,Wenxian Tu,Yunfeng Wang,Yuanmin Du,Kang Xu
View all publications
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Product promise

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