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Rabbit Recombinant Monoclonal MED4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.


Images

Western blot - Anti-MED4 antibody [EPR7816] (AB129170), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (AB129170), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (AB129170), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (AB129170), expandable thumbnail
  • OI-RD Scanning - Anti-MED4 antibody [EPR7816] (AB129170), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat, Xenopus laevis
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Xenopus laevis
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Xenopus laevis
Dilution info
-
Notes

-

Target data

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Alternative names

ARC36, DRIP36, VDRIP, HSPC126, MED4, Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component

Recommended products

Rabbit Recombinant Monoclonal MED4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7816
Purification technique
Affinity purification Protein A
Dissociation constant
1.43 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MED4 also known as mediator complex subunit 4 plays an important role in transcription regulation. MED4 forms part of the larger mediator complex which interacts with RNA polymerase II during the transcription initiation process. This protein has a mass of approximately 35 kilodaltons. Researchers have detected MED4 expression in various tissues with notable levels in the liver and lung implying its broad involvement in different cellular processes.

Biological function summary

MED4 operates as an integral component of the mediator complex a multi-protein machinery that transmits signals from transcription factors to RNA polymerase II facilitating the transcription of specific genes. This mediator complex acts as a bridge affecting transcription initiation and further steps. MED4's presence within this complex highlights its importance in regulating gene expression and maintaining normal cell function.

Pathways

MED4 directly contributes to the RNA polymerase II transcription initiation complex pathway. This pathway is important for transcribing genes into mRNA. MED4 also engages in chromatin-related processes through its mediator role influencing the transcription machinery's interaction with chromatin structures. Connections between MED4 and other proteins like MED1 and MED12 highlight its collaborative function in transcription regulation.

Associated diseases and disorders

Abnormal MED4 function associates with certain types of cancer including breast cancer. Research suggests that dysfunctional MED4 can disrupt typical transcriptional regulation leading to uncontrolled cell proliferation. Additionally alterations in MED4 have links to neurological disorders as they may affect gene expression necessary for normal brain function. MED4's relationship with proteins like p53 in cancer further highlights its role in disease pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-MED4 antibody [EPR7816] (ab129170), expandable thumbnail

    Western blot - Anti-MED4 antibody [EPR7816] (ab129170)

    All lanes: Western blot - Anti-MED4 antibody [EPR7816] (ab129170) at 1/1000 dilution

    Lane 1: HepG2 cell lysates at 10 µg

    Lane 2: T-47D cell lysates at 10 µg

    Lane 3: K562 cell lysates at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)

    ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human breast carcinoma tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (ab129170), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (ab129170)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling MED4 with ab129170 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)

    ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-MED4 antibody [EPR7816] (ab129170), expandable thumbnail

    OI-RD Scanning - Anti-MED4 antibody [EPR7816] (ab129170)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-MED4 antibody [EPR7816] (ab129170), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-MED4 antibody [EPR7816] (ab129170)

    MED4 western blot using anti-MED4 antibody [EPR7816] ab129170. Publication image and figure legend from Zhang, N., Song, Y., et al., 2020, Theranostics, PubMed 32802198.


    ab129170 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab129170 please see the product overview.

    MED13L bridges the CDK8 kinase module with the core Mediator. (A, B) Immunoprecipitation (IP) experiments using antibodies to MED13L, MED6, MED8, MED1, MED4, MED23, MED12, CCNC and CDK8 in PC9 and A549 cells. (C) A carton model indicating the role of MED13L as a vital linker between the core Mediator and the kinase module. (D) IP experiments between P300, MED13L and MED12 in NSCLC cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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