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AB129170

Anti-MED4 antibody [EPR7816]

5

(2 Reviews)

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(8 Publications)

Rabbit Recombinant Monoclonal MED4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

ARC36, DRIP36, VDRIP, HSPC126, MED4, Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (AB129170)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (AB129170)

ab129170 at 1/100 dilution staining MED4 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (AB129170)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (AB129170)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling MED4 with ab129170 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control : PBS only.
Nuclear counter stain : DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (AB129170)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (AB129170)

ab129170 at 1/100 dilution staining MED4 in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-MED4 antibody [EPR7816] (AB129170)
  • WB

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Western blot - Anti-MED4 antibody [EPR7816] (AB129170)

All lanes:

Western blot - Anti-MED4 antibody [EPR7816] (ab129170) at 1/1000 dilution

Lane 1:

HepG2 cell lysates at 10 µg

Lane 2:

T-47D cell lysates at 10 µg

Lane 3:

K562 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 30 kDa

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OI-RD Scanning - Anti-MED4 antibody [EPR7816] (AB129170)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MED4 antibody [EPR7816] (AB129170)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Western blot - Anti-MED4 antibody [EPR7816] (AB129170)
  • WB

CiteAb

Western blot - Anti-MED4 antibody [EPR7816] (AB129170)

MED4 western blot using anti-MED4 antibody [EPR7816] ab129170. Publication image and figure legend from Zhang, N., Song, Y., et al., 2020, Theranostics, PubMed 32802198.

ab129170 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab129170 please see the product overview.

MED13L bridges the CDK8 kinase module with the core Mediator. (A, B) Immunoprecipitation (IP) experiments using antibodies to MED13L, MED6, MED8, MED1, MED4, MED23, MED12, CCNC and CDK8 in PC9 and A549 cells. (C) A carton model indicating the role of MED13L as a vital linker between the core Mediator and the kinase module. (D) IP experiments between P300, MED13L and MED12 in NSCLC cells.

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  • Carrier free

    Anti-MED4 antibody [EPR7816] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7816

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED4 also known as mediator complex subunit 4 plays an important role in transcription regulation. MED4 forms part of the larger mediator complex which interacts with RNA polymerase II during the transcription initiation process. This protein has a mass of approximately 35 kilodaltons. Researchers have detected MED4 expression in various tissues with notable levels in the liver and lung implying its broad involvement in different cellular processes.
Biological function summary

MED4 operates as an integral component of the mediator complex a multi-protein machinery that transmits signals from transcription factors to RNA polymerase II facilitating the transcription of specific genes. This mediator complex acts as a bridge affecting transcription initiation and further steps. MED4's presence within this complex highlights its importance in regulating gene expression and maintaining normal cell function.

Pathways

MED4 directly contributes to the RNA polymerase II transcription initiation complex pathway. This pathway is important for transcribing genes into mRNA. MED4 also engages in chromatin-related processes through its mediator role influencing the transcription machinery's interaction with chromatin structures. Connections between MED4 and other proteins like MED1 and MED12 highlight its collaborative function in transcription regulation.

Abnormal MED4 function associates with certain types of cancer including breast cancer. Research suggests that dysfunctional MED4 can disrupt typical transcriptional regulation leading to uncontrolled cell proliferation. Additionally alterations in MED4 have links to neurological disorders as they may affect gene expression necessary for normal brain function. MED4's relationship with proteins like p53 in cancer further highlights its role in disease pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED4
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Retrovirology 21:13 PubMed38898526

2024

Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory S Lambert,Breanna L Rice,Rebecca J Kaddis Maldonado,Jordan Chang,Leslie J Parent

Journal of virology 97:e0117923 PubMed37991367

2023

HIV-1 Gag co-localizes with euchromatin histone marks at the nuclear periphery.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan Chang,Leslie J Parent

Cell genomics 3:100250 PubMed36950384

2023

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper Lage

Molecular cell 83:186-202.e11 PubMed36669479

2023

PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII.

Applications

Unspecified application

Species

Unspecified reactive species

Xavier Rambout,Hana Cho,Roméo Blanc,Qing Lyu,Joseph M Miano,Joe V Chakkalakal,Geoffrey M Nelson,Hari K Yalamanchili,Karen Adelman,Lynne E Maquat

Cell 186:327-345.e28 PubMed36603581

2023

Functional partitioning of transcriptional regulators by patterned charge blocks.

Applications

Unspecified application

Species

Unspecified reactive species

Heankel Lyons,Reshma T Veettil,Prashant Pradhan,Christy Fornero,Nancy De La Cruz,Keiichi Ito,Mikayla Eppert,Robert G Roeder,Benjamin R Sabari

Viruses 12: PubMed33182496

2020

HIV-1 Gag Forms Ribonucleoprotein Complexes with Unspliced Viral RNA at Transcription Sites.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin M Tuffy,Rebecca J Kaddis Maldonado,Jordan Chang,Paul Rosenfeld,Alan Cochrane,Leslie J Parent

Theranostics 10:9378-9394 PubMed32802198

2020

MED13L integrates Mediator-regulated epigenetic control into lung cancer radiosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Nasha Zhang,Yemei Song,Yeyang Xu,Jiandong Liu,Yue Shen,Liqing Zhou,Jinming Yu,Ming Yang

Frontiers in immunology 9:2879 PubMed30574148

2018

The C-Terminal Transactivation Domain of STAT1 Has a Gene-Specific Role in Transactivation and Cofactor Recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Parrini,Katrin Meissl,Mojoyinola Joanna Ola,Therese Lederer,Ana Puga,Sebastian Wienerroither,Pavel Kovarik,Thomas Decker,Mathias Müller,Birgit Strobl
View all publications
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