Rabbit Recombinant Monoclonal MED4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--western-blot-img106483.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128589.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327)")
![Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--immunocytochemistry-immunofluorescence-img41765.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115174.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327)")
![OI-RD Scanning - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--oi-rd-scanning-img142413.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-MED4 antibody [EPR7816] - BSA and Azide free (AB248327)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis | Dilution info - | Notes - |
Target data
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alternative names
ARC36, DRIP36, VDRIP, HSPC126, MED4, Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component
Recommended products
Rabbit Recombinant Monoclonal MED4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7816
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.43 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248327 is the carrier-free version of Anti-MED4 antibody [EPR7816] ab129170.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MED4 also known as mediator complex subunit 4 plays an important role in transcription regulation. MED4 forms part of the larger mediator complex which interacts with RNA polymerase II during the transcription initiation process. This protein has a mass of approximately 35 kilodaltons. Researchers have detected MED4 expression in various tissues with notable levels in the liver and lung implying its broad involvement in different cellular processes.
- Biological function summary
MED4 operates as an integral component of the mediator complex a multi-protein machinery that transmits signals from transcription factors to RNA polymerase II facilitating the transcription of specific genes. This mediator complex acts as a bridge affecting transcription initiation and further steps. MED4's presence within this complex highlights its importance in regulating gene expression and maintaining normal cell function.
- Pathways
MED4 directly contributes to the RNA polymerase II transcription initiation complex pathway. This pathway is important for transcribing genes into mRNA. MED4 also engages in chromatin-related processes through its mediator role influencing the transcription machinery's interaction with chromatin structures. Connections between MED4 and other proteins like MED1 and MED12 highlight its collaborative function in transcription regulation.
- Associated diseases and disorders
Abnormal MED4 function associates with certain types of cancer including breast cancer. Research suggests that dysfunctional MED4 can disrupt typical transcriptional regulation leading to uncontrolled cell proliferation. Additionally alterations in MED4 have links to neurological disorders as they may affect gene expression necessary for normal brain function. MED4's relationship with proteins like p53 in cancer further highlights its role in disease pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--western-blot-img106483.jpg "Western blot - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)")
Western blot - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
This data was developed using Anti-MED4 antibody [EPR7816] ab129170, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-MED4 antibody [EPR7816] (Anti-MED4 antibody [EPR7816] ab129170) at 1/1000 dilution
Lane 1: HepG2 cell lysates at 10 µg
Lane 2: T-47D cell lysates at 10 µg
Lane 3: K562 cell lysates at 10 µg
SecondaryAll lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128589.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
This data was developed using Anti-MED4 antibody [EPR7816] ab129170, the same antibody clone in a different buffer formulation.Anti-MED4 antibody [EPR7816] ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human breast carcinoma tissue, by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--immunocytochemistry-immunofluorescence-img41765.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)")
Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
This data was developed using Anti-MED4 antibody [EPR7816] ab129170, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling MED4 with Anti-MED4 antibody [EPR7816] ab129170 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115174.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
This data was developed using Anti-MED4 antibody [EPR7816] ab129170, the same antibody clone in a different buffer formulation.Anti-MED4 antibody [EPR7816] ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human colon tissue, by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327), expandable thumbnail](https://content.abcam.com/products/images/med4-antibody-epr7816-bsa-and-azide-free-ab248327--oi-rd-scanning-img142413.jpg "OI-RD Scanning - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)")
OI-RD Scanning - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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