Rabbit Polyclonal MED6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MED6 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MED6 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O75586
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alternative names
ARC33, MED6, Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, Mediator complex subunit 6, Renal carcinoma antigen NY-REN-28, hMed6
Recommended products
Rabbit Polyclonal MED6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MED6 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MED6 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O75586
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MED6 also known as Mediator complex subunit 6 is an integral component of the Mediator complex which is essential in the transcription regulation. MED6 has a molecular weight of approximately 26 kDa and can be found expressed in various tissues including liver and kidney. MED6 assists in bridging the regulatory molecules to RNA polymerase II machinery helping control the transcription of specific genes.
- Biological function summary
MED6 acts as a component of the larger Mediator complex connecting transcription factors with the RNA polymerase II enzyme. This connection facilitates transcription initiation and regulation processes. The complex structure of the Mediator allows proper assembly with other essential proteins to regulate transcription effectively contributing to important cellular activities.
- Pathways
The activity of the MED6 protein aligns closely with key biological pathways such as the Wnt signaling pathway and the MAPK signaling pathway. MED6 operates in concert with other Mediator complex subunits to modulate these pathways coordinating with proteins like beta-catenin in Wnt signaling and ERK proteins in MAPK signaling emphasizing its impact in cellular growth and differentiation processes.
- Associated diseases and disorders
Abnormalities in the function or expression of MED6 have been linked to various cancers including colorectal cancer. Changes in its function can disrupt normal transcription processes influencing the expression of genes critical in tumor progression. Additionally MED6's interactions with other proteins such as those involved in the transcription regulation like p53 can further exemplify its connection to oncogenesis underlining potential therapeutic targets for disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-MED6 antibody (ab220110)
Immunofluorescent analysis of PFA fixed, Triton X-100 permeabilized U-2 OS cells labeling MED6 with ab220110 at 4 μg/ml (green).
Western blot - Anti-MED6 antibody (ab220110)
All lanes: Western blot - Anti-MED6 antibody (ab220110) at 1/100 dilution
All lanes: RT-4 cell lysate
Predicted band size: 28 kDa
Downloads
Product protocols
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