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AB220110

Anti-MED6 antibody

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(2 Publications)

Rabbit Polyclonal MED6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MED6 aa 1-200.

View Alternative Names

ARC33, MED6, Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, Mediator complex subunit 6, Renal carcinoma antigen NY-REN-28, hMed6

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MED6 antibody (AB220110)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MED6 antibody (AB220110)

Immunofluorescent analysis of PFA fixed, Triton X-100 permeabilized U-2 OS cells labeling MED6 with ab220110 at 4 μg/ml (green).

Western blot - Anti-MED6 antibody (AB220110)
  • WB

Supplier Data

Western blot - Anti-MED6 antibody (AB220110)

All lanes:

Western blot - Anti-MED6 antibody (ab220110) at 1/100 dilution

All lanes:

RT-4 cell lysate

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MED6 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O75586

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED6 also known as Mediator complex subunit 6 is an integral component of the Mediator complex which is essential in the transcription regulation. MED6 has a molecular weight of approximately 26 kDa and can be found expressed in various tissues including liver and kidney. MED6 assists in bridging the regulatory molecules to RNA polymerase II machinery helping control the transcription of specific genes.
Biological function summary

MED6 acts as a component of the larger Mediator complex connecting transcription factors with the RNA polymerase II enzyme. This connection facilitates transcription initiation and regulation processes. The complex structure of the Mediator allows proper assembly with other essential proteins to regulate transcription effectively contributing to important cellular activities.

Pathways

The activity of the MED6 protein aligns closely with key biological pathways such as the Wnt signaling pathway and the MAPK signaling pathway. MED6 operates in concert with other Mediator complex subunits to modulate these pathways coordinating with proteins like beta-catenin in Wnt signaling and ERK proteins in MAPK signaling emphasizing its impact in cellular growth and differentiation processes.

Abnormalities in the function or expression of MED6 have been linked to various cancers including colorectal cancer. Changes in its function can disrupt normal transcription processes influencing the expression of genes critical in tumor progression. Additionally MED6's interactions with other proteins such as those involved in the transcription regulation like p53 can further exemplify its connection to oncogenesis underlining potential therapeutic targets for disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 56:1654-1664 PubMed39048795

2024

Cohesin prevents cross-domain gene coactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Dong,Shu Zhang,Valentina Gandin,Liangqi Xie,Lihua Wang,Andrew L Lemire,Wenhong Li,Hideo Otsuna,Takashi Kawase,Arthur D Lander,Howard Y Chang,Zhe J Liu

Genes to cells : devoted to molecular & cellular mechanisms 24:284-296 PubMed30762924

2019

Spironolactone-induced XPB degradation depends on CDK7 kinase and SCF E3 ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Masanobu Ueda,Kenkyo Matsuura,Hidehiko Kawai,Mitsuo Wakasugi,Tsukasa Matsunaga
View all publications
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