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AB249702

Anti-MED6 antibody [EPR12107] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MED6 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ARC33, MED6, Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, Mediator complex subunit 6, Renal carcinoma antigen NY-REN-28, hMed6

1 Images
Western blot - Anti-MED6 antibody [EPR12107] - BSA and Azide free (AB249702)
  • WB

Supplier Data

Western blot - Anti-MED6 antibody [EPR12107] - BSA and Azide free (AB249702)

This data was developed using ab172605, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MED6 antibody [EPR12107] (<a href='/en-us/products/primary-antibodies/med6-antibody-epr12107-ab172605'>ab172605</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

HepG2 cell lysates at 10 µg

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12107

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249702 is the carrier-free version of ab172605.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED6 also known as Mediator complex subunit 6 is an integral component of the Mediator complex which is essential in the transcription regulation. MED6 has a molecular weight of approximately 26 kDa and can be found expressed in various tissues including liver and kidney. MED6 assists in bridging the regulatory molecules to RNA polymerase II machinery helping control the transcription of specific genes.
Biological function summary

MED6 acts as a component of the larger Mediator complex connecting transcription factors with the RNA polymerase II enzyme. This connection facilitates transcription initiation and regulation processes. The complex structure of the Mediator allows proper assembly with other essential proteins to regulate transcription effectively contributing to important cellular activities.

Pathways

The activity of the MED6 protein aligns closely with key biological pathways such as the Wnt signaling pathway and the MAPK signaling pathway. MED6 operates in concert with other Mediator complex subunits to modulate these pathways coordinating with proteins like beta-catenin in Wnt signaling and ERK proteins in MAPK signaling emphasizing its impact in cellular growth and differentiation processes.

Abnormalities in the function or expression of MED6 have been linked to various cancers including colorectal cancer. Changes in its function can disrupt normal transcription processes influencing the expression of genes critical in tumor progression. Additionally MED6's interactions with other proteins such as those involved in the transcription regulation like p53 can further exemplify its connection to oncogenesis underlining potential therapeutic targets for disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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