AB250909

Anti-MED7 antibody [EPR15410] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MED7 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

ARC34, CRSP9, MED7, Mediator of RNA polymerase II transcription subunit 7, hMED7, Activator-recruited cofactor 34 kDa component, Cofactor required for Sp1 transcriptional activation subunit 9, Mediator complex subunit 7, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33, CRSP complex subunit 9

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

This data was developed using ab187146, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

This data was developed using ab187146, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of HeLa cells labeling MED7 with ab187146 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

This data was developed using ab187146, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

This data was developed using ab187146, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MED7 antibody [EPR15410] (<a href='/en-us/products/primary-antibodies/med7-antibody-epr15410-ab187146'>ab187146</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

This data was developed using ab187146, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MED7 antibody [EPR15410] (<a href='/en-us/products/primary-antibodies/med7-antibody-epr15410-ab187146'>ab187146</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15410

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250909 is the carrier-free version of ab187146.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MED7 also known as Mediator Complex Subunit 7 is a protein component of core Mediator complex essential for transcription regulation by RNA polymerase II. MED7 weighs approximately 25 kDa and exists in nucleus. It is part of a large multi-protein assembly. This subunit participates specifically by aiding in the mediator's interactions with gene-specific regulatory proteins and general transcription factors essential for transcription initiation.

Biological function summary

These components coordinate as part of Mediator complex which regulates transcription by acting as bridge between DNA-bound transcriptional regulators and RNA polymerase II. MED7 contributes to the structural integrity of Mediator complex affecting transcriptional activation processes necessary for gene expression. The protein's roles link it directly to cell growth differentiation and response to various stimuli indicating its importance in diverse cellular processes.

Pathways

MED7 integrates itself into transcription regulation pathways especially interacting with other subunits like MED1 and MED14. The Mediator complex functions in the pathway responsible for transferring information from transcription factors to RNA polymerase II. This involves interacting with multiple pathways including Notch signaling and Wnt signaling serving as coordinator between transcriptional regulators and transcription machinery.

MED7 has associations with cancer and neurodevelopmental disorders. Aberrations in Mediator complex components including MED7 can impact transcriptional dysregulation linked to oncogenesis. Mutations in this subunit along with changes in associated proteins such as MED12 are observed in various cancer types. Additionally disturbances in Mediator complex function potentially involving MED7 contribute to neurodevelopmental conditions due to misregulation of gene expression essential for neural development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

See full target information MED7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MED7 antibody [EPR15410] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

Western blot - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

Western blot - Anti-MED7 antibody [EPR15410] - BSA and Azide free (AB250909)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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