Rabbit Polyclonal MEF2A antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MEF2A.
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Publications
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- Orphanet journal of rare diseases 18:2662023HDAC5 inhibition attenuates ventricular remodeling and cardiac dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenxi Zhu,Zhehao Piao,Li JinPubMed 37667300
- Bone 138:1154662020Loss of myocyte enhancer factor 2 expression in osteoclasts leads to opposing skeletal phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas Blixt et. al.PubMed 32512162
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MEF2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q02078
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Tested | Tested | Expected |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.
Alternative names
MEF2, MEF2A, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1
Recommended products
Rabbit Polyclonal MEF2A antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MEF2A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MEF2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q02078
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEF2A or Myocyte Enhancer Factor 2A is a transcription factor involved in regulating gene expression. MEF2A is approximately 55 kDa in size and is expressed in a variety of tissues including muscle brain and heart. It is part of the MEF2 family of transcription factors which all share a similar MADS-box/MEF2 domain structure. MEF2A binds to DNA and influences the transcription of genes involved in muscle differentiation and development.
- Biological function summary
MEF2A participates in transcriptional regulation as part of a larger complex that includes other regulatory proteins. This transcription factor plays an essential role in the development and function of muscles. It activates the transcription of genes necessary for muscle fiber formation and adaptation. MEF2A also influences the expression of genes related to neuronal survival and plasticity impacting cognitive function and memory in the brain.
- Pathways
MEF2A is involved in both the MAPK/ERK and PI3K/Akt pathways which are significant for cellular growth survival and differentiation. MEF2A interacts with proteins like MAPK and PI3K impacting these signaling pathways that regulate cell proliferation and apoptosis. This interaction highlights its role in cellular response to external stimuli and stress signals positioning MEF2A as an important factor in cell fate decisions.
- Associated diseases and disorders
Mutations or dysregulation of MEF2A are connected to cardiovascular diseases particularly coronary artery disease. MEF2A mutations can disrupt normal transcriptional regulation leading to impaired cardiovascular function. Additionally alterations in MEF2A function are linked to neurodegenerative disorders such as Alzheimer's disease where it may interact with proteins including Tau and amyloid precursor protein influencing neuronal health and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-MEF2A antibody - C-terminal (ab227120)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-MEF2A antibody - C-terminal (ab227120) at 1/500 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell extract at 30 µg
Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A antibody - C-terminal (ab227120)
Paraffin-embedded mouse fore brain tissue stained for MEF2A using ab227120 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A antibody - C-terminal (ab227120)
Paraffin-embedded rat fore brain tissue stained for MEF2A using ab227120 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody - C-terminal (ab227120)
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MEF2A (green) using ab227120 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
Western blot - Anti-MEF2A antibody - C-terminal (ab227120)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-MEF2A antibody - C-terminal (ab227120) at 1/1000 dilution
All lanes: NCI-H1299 whole cell lysate at 30 µg
Predicted band size: 55 kDa
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