AB227120

Anti-MEF2A antibody - C-terminal

Name: Anti-MEF2A antibody - C-terminal (ab227120) | Abcam
Brand: Abcam Limited
SKU: AB227120
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal MEF2A antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MEF2A.

View Alternative Names

MEF2, MEF2A, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody - C-terminal (AB227120)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody - C-terminal (AB227120)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MEF2A (green) using ab227120 at 1/200 dilution in ICC/IF. Nuclear Counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A antibody - C-terminal (AB227120)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A antibody - C-terminal (AB227120)

Paraffin-embedded mouse fore brain tissue stained for MEF2A using ab227120 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A antibody - C-terminal (AB227120)

Paraffin-embedded rat fore brain tissue stained for MEF2A using ab227120 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MEF2A antibody - C-terminal (AB227120)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-MEF2A antibody - C-terminal (ab227120) at 1/1000 dilution

All lanes:

NCI-H1299 whole cell lysate at 30 µg

Predicted band size: 55 kDa

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Supplier Data

Western blot - Anti-MEF2A antibody - C-terminal (AB227120)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-MEF2A antibody - C-terminal (ab227120) at 1/500 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell extract at 30 µg

Predicted band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MEF2A. The exact immunogen used to generate this antibody is proprietary information.

Q02078

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEF2A or Myocyte Enhancer Factor 2A is a transcription factor involved in regulating gene expression. MEF2A is approximately 55 kDa in size and is expressed in a variety of tissues including muscle brain and heart. It is part of the MEF2 family of transcription factors which all share a similar MADS-box/MEF2 domain structure. MEF2A binds to DNA and influences the transcription of genes involved in muscle differentiation and development.

Biological function summary

MEF2A participates in transcriptional regulation as part of a larger complex that includes other regulatory proteins. This transcription factor plays an essential role in the development and function of muscles. It activates the transcription of genes necessary for muscle fiber formation and adaptation. MEF2A also influences the expression of genes related to neuronal survival and plasticity impacting cognitive function and memory in the brain.

Pathways

MEF2A is involved in both the MAPK/ERK and PI3K/Akt pathways which are significant for cellular growth survival and differentiation. MEF2A interacts with proteins like MAPK and PI3K impacting these signaling pathways that regulate cell proliferation and apoptosis. This interaction highlights its role in cellular response to external stimuli and stress signals positioning MEF2A as an important factor in cell fate decisions.

Mutations or dysregulation of MEF2A are connected to cardiovascular diseases particularly coronary artery disease. MEF2A mutations can disrupt normal transcriptional regulation leading to impaired cardiovascular function. Additionally alterations in MEF2A function are linked to neurodegenerative disorders such as Alzheimer's disease where it may interact with proteins including Tau and amyloid precursor protein influencing neuronal health and survival.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.

See full target information MEF2A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Orphanet journal of rare diseases 18:266 PubMed37667300

2023

HDAC5 inhibition attenuates ventricular remodeling and cardiac dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Zhu,Zhehao Piao,Li Jin

Bone 138:115466 PubMed32512162

2020

Loss of myocyte enhancer factor 2 expression in osteoclasts leads to opposing skeletal phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Blixt,Andrew Norton,Anqi Zhang,Conrado Aparicio,Hari Prasad,Rajaram Gopalakrishnan,Eric D Jensen,Kim C Mansky

eLife 5: PubMed27763263

2016

Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gao,Gülce Sila Gülcüler,Lieke Golbach,Helena Block,Alexander Zarbock,Ana Martin-Villalba

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEF2A antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody - C-terminal (AB227120)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A antibody - C-terminal (AB227120)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A antibody - C-terminal (AB227120)

Western blot - Anti-MEF2A antibody - C-terminal (AB227120)

Western blot - Anti-MEF2A antibody - C-terminal (AB227120)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise