Rabbit Recombinant Monoclonal MEF2A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications.
Images
![Western blot - Anti-MEF2A antibody [EPR1451] (AB109420), expandable thumbnail](https://content.abcam.com/products/images/mef2a-antibody-epr1451-ab109420--western-blot-img112913.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MEF2A antibody [EPR1451] (AB109420)")
![Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EPR1451] (AB109420), expandable thumbnail](https://content.abcam.com/products/images/mef2a-antibody-epr1451-ab109420--immunocytochemistry-immunofluorescence-img11337.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EPR1451] (AB109420)")
Publications
Filter by
- Frontiers in bioengineering and biotechnology 10:10228302022Hsa_circ_0008870 suppresses bone formation of growth plate through inhibition of miR-185-3p/ MAPK1 axis in idiopathic short stature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiwen Wu,Jinghong Yuan,Jiantian Li,Zhi Du,Ming Yin,Xigao Cheng,Xijuan Liu,Jingyu JiaPubMed 36304901
- Frontiers in cell and developmental biology 9:6718572021Lnc-GD2H Promotes Proliferation by Forming a Feedback Loop With c-Myc and Enhances Differentiation Through Interacting With NACA to Upregulate Myog in C2C12 Myoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Chen,Si Lei,Yanling She,Shanyao Zhou,Huacai Shi,Cheng Li,Ting JiangPubMed 34490239
- Frontiers in physiology 12:6592722021Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanling She,Cheng Li,Ting Jiang,Si Lei,Shanyao Zhou,Huacai Shi,Rui ChenPubMed 34305633
- Nature communications 10:32762019Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie Payne et. al.PubMed 31332177
- The Journal of neuroscience : the official journal 39:5064-50792019Antagonizing Increased Levels at the Chronic Stage of Experimental TLE Reduces Spontaneous Recurrent Seizures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVamshidhar R Vangoor et. al.PubMed 31015341
- Biochemical and biophysical research communication 500:645-6492018The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBangfu Zhu et. al.PubMed 29678571
- Scientific reports 8:52632018MEF2A regulates mGluR-dependent AMPA receptor trafficking independently of Arc/Arg3.1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuth E Carmichael et. al.PubMed 29588465
- Genes & development 30:2297-23092016MEF2 transcription factors are key regulators of sprouting angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Sacilotto,Kira M Chouliaras,Leonid L Nikitenko,Yao Wei Lu,Martin Fritzsche,Marsha D Wallace,Svanhild Nornes,Fernando García-Moreno,Sophie Payne,Esther Bridges,Ke Liu,Daniel Biggs,Indrika Ratnayaka,Shane P Herbert,Zoltán Molnár,Adrian L Harris,Benjamin Davies,Gareth L Bond,George Bou-Gharios,John J Schwarz,Sarah De ValPubMed 27898394
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.
Alternative names
MEF2, MEF2A, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1
Recommended products
Rabbit Recombinant Monoclonal MEF2A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR1451
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEF2A or Myocyte Enhancer Factor 2A is a transcription factor involved in regulating gene expression. MEF2A is approximately 55 kDa in size and is expressed in a variety of tissues including muscle brain and heart. It is part of the MEF2 family of transcription factors which all share a similar MADS-box/MEF2 domain structure. MEF2A binds to DNA and influences the transcription of genes involved in muscle differentiation and development.
- Biological function summary
MEF2A participates in transcriptional regulation as part of a larger complex that includes other regulatory proteins. This transcription factor plays an essential role in the development and function of muscles. It activates the transcription of genes necessary for muscle fiber formation and adaptation. MEF2A also influences the expression of genes related to neuronal survival and plasticity impacting cognitive function and memory in the brain.
- Pathways
MEF2A is involved in both the MAPK/ERK and PI3K/Akt pathways which are significant for cellular growth survival and differentiation. MEF2A interacts with proteins like MAPK and PI3K impacting these signaling pathways that regulate cell proliferation and apoptosis. This interaction highlights its role in cellular response to external stimuli and stress signals positioning MEF2A as an important factor in cell fate decisions.
- Associated diseases and disorders
Mutations or dysregulation of MEF2A are connected to cardiovascular diseases particularly coronary artery disease. MEF2A mutations can disrupt normal transcriptional regulation leading to impaired cardiovascular function. Additionally alterations in MEF2A function are linked to neurodegenerative disorders such as Alzheimer's disease where it may interact with proteins including Tau and amyloid precursor protein influencing neuronal health and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-MEF2A antibody [EPR1451] (ab109420), expandable thumbnail](https://content.abcam.com/products/images/mef2a-antibody-epr1451-ab109420--western-blot-img112913.jpg "Western blot - Anti-MEF2A antibody [EPR1451] (ab109420)")
Western blot - Anti-MEF2A antibody [EPR1451] (ab109420)
All lanes: Western blot - Anti-MEF2A antibody [EPR1451] (ab109420) at 1/10000 dilution
Lane 1: Human heart lysate at 10 µg
Lane 2: Human skeletal muscle lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: Saos-2 cell lysate at 10 µg
Predicted band size: 55 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EPR1451] (ab109420), expandable thumbnail](https://content.abcam.com/products/images/mef2a-antibody-epr1451-ab109420--immunocytochemistry-immunofluorescence-img11337.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EPR1451] (ab109420)")
Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EPR1451] (ab109420)
ab109420, at 1/100 dilution, staining MEF2A in HeLa cells by Immunohistochemistry.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com