AB282242

Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MEF2C antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Myocyte-specific enhancer factor 2C, Myocyte enhancer factor 2C, MEF2C

3 Images

Sandwich ELISA - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector) (AB282242)

sELISA

Lab

Sandwich ELISA - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector) (AB282242)

Interpolated concentrations of native MEF2C in human control wild type THP-1 (human acute monocytic leukemia cell) and MEF2C knockout THP-1 cell based on 125 µg/mL extract loads. The concentrations of MEF2C were measured in duplicate and interpolated from the MEF2C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MEF2C concentration was determined to be 9471.1 pg/mL in wild type THP-1 extract (Human wild-type THP-1 cell line ab281894) and undetectable in MEF2C knockout THP-1 extract (Human MEF2C knockout THP-1 cell line ab313739).

sELISA

Supplier Data

Sandwich ELISA - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector) (AB282242)

Example of Human MEF2C standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector) (AB282242)

Example of Human MEF2C standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Unconjugated

Anti-MEF2A + MEF2C antibody [EPR19089-34]
Carrier free

Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19089-34

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab282242 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MEF2A and MEF2C. The recommended pair for sandwich ELISA is:
Capture: ab282243, Human MEF2C Capture Antibody (unconjugated)
Detector: ab282242, Human MEF2C Detector Antibody (unconjugated)
The reference range value is 0.078 - 20 ng/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEF2A (Myocyte Enhancer Factor 2A) and MEF2C (Myocyte Enhancer Factor 2C) are transcription factors belonging to the MEF2 family. MEF2A has a molecular mass of approximately 55 kDa while MEF2C is around 51 kDa. Both MEF2A and MEF2C modulate gene expression through direct binding to regulatory DNA sequences. These proteins express in numerous tissues including muscle brain and vascular tissues indicating their extensive role in various cellular processes. MEF2A and MEF2C closely relate due to their structural similarities and functional overlap often working in concert with similar cofactors and regulatory proteins.

Biological function summary

MEF2A and MEF2C regulate multiple cellular processes especially in muscle differentiation and neuronal development. They frequently form complexes with other transcriptional regulators and play a significant role in the activation and repression of target genes. Through their interaction with the regulatory regions of DNA they help coordinate the transcriptional response necessary for cell growth development and survival. Their important involvement in muscle and nervous system development positions them as central players in maintaining proper cellular function during these processes.

Pathways

MEF2A and MEF2C serve as integral components in the MAPK and calcium signaling pathways. These pathways impact cellular response to environmental stimuli and regulate critical processes such as cell growth and differentiation. Interacting proteins like HDACs (histone deacetylases) further modulate the activity of MEF2A and MEF2C affecting their ability to regulate gene expression in response to various signals. This relationship is essential because it sustains the balance between activation and repression of target genes within these pathways.

MEF2A and MEF2C have significant implications in cardiovascular diseases and neurological disorders. Mutations or dysregulation in MEF2A often link to coronary artery disease highlighting its importance in cardiovascular health. Meanwhile MEF2C plays a role in autism spectrum disorders with abnormalities in its expression or function disrupting normal neuronal development. Both MEF2A and MEF2C interact with proteins such as GATA4 in the context of cardiac function and synaptic proteins for neuronal development respectively providing potential therapeutic targets for addressing the associated diseases and disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoforms that lack the repressor domain are more active than isoform 1.

See full target information MEF2C

Additional targets

MEF2A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector) (AB282242)

Sandwich ELISA - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector) (AB282242)

Sandwich ELISA - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (Detector) (AB282242)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise