Rabbit Polyclonal MEF2A phospho T312 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MEF2A phospho T312.
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- Redox biology 36:1016692020Oxidation of HDAC4 by Nox4-derived HO maintains tube formation by endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTim Schader et. al.PubMed 32818796
- Journal of clinical biochemistry and nutrition 61:6-132017CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtsuko Ohashi,Hiroyuki Yasuda,Tetsuro Kamiya,Hirokazu Hara,Tetsuo AdachiPubMed 28751803
- The Biochemical journal 459:427-392014A novel p38 MAPK docking-groove-targeted compound is a potent inhibitor of inflammatory hyperalgesia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanneke L D M Willemen,Pedro M Campos,Elisa Lucas,Antonio Morreale,Rubén Gil-Redondo,Juan Agut,Florenci V González,Paula Ramos,Cobi Heijnen,Federico Mayor,Annemieke Kavelaars,Cristina MurgaPubMed 24517375
- Arteriosclerosis, thrombosis, and vascular biology 29:1902-82009Flow activation of AMP-activated protein kinase in vascular endothelium leads to Krüppel-like factor 2 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela Young et. al.PubMed 19696400
- The Journal of neuroscience : the official journal of the Society for Neuroscience 29:6700-92009A retrograde neuronal survival response: target-derived neurotrophins regulate MEF2D and bcl-w.Applications:WBReactive species:RatMaria F Pazyra-Murphy,Aymeric Hans,Stephanie L Courchesne,Christoph Karch,Katharina E Cosker,Heather M Heerssen,Fiona L Watson,Taekyung Kim,Michael E Greenberg,Rosalind A SegalPubMed 19458239
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MEF2A phospho T312. The exact immunogen used to generate this antibody is proprietary information. Database link Q02078
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.
Alternative names
MEF2, MEF2A, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1
Recommended products
Rabbit Polyclonal MEF2A phospho T312 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MEF2A phospho T312.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MEF2A phospho T312. The exact immunogen used to generate this antibody is proprietary information. Database link Q02078
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEF2A or Myocyte Enhancer Factor 2A is a transcription factor involved in regulating gene expression. MEF2A is approximately 55 kDa in size and is expressed in a variety of tissues including muscle brain and heart. It is part of the MEF2 family of transcription factors which all share a similar MADS-box/MEF2 domain structure. MEF2A binds to DNA and influences the transcription of genes involved in muscle differentiation and development.
- Biological function summary
MEF2A participates in transcriptional regulation as part of a larger complex that includes other regulatory proteins. This transcription factor plays an essential role in the development and function of muscles. It activates the transcription of genes necessary for muscle fiber formation and adaptation. MEF2A also influences the expression of genes related to neuronal survival and plasticity impacting cognitive function and memory in the brain.
- Pathways
MEF2A is involved in both the MAPK/ERK and PI3K/Akt pathways which are significant for cellular growth survival and differentiation. MEF2A interacts with proteins like MAPK and PI3K impacting these signaling pathways that regulate cell proliferation and apoptosis. This interaction highlights its role in cellular response to external stimuli and stress signals positioning MEF2A as an important factor in cell fate decisions.
- Associated diseases and disorders
Mutations or dysregulation of MEF2A are connected to cardiovascular diseases particularly coronary artery disease. MEF2A mutations can disrupt normal transcriptional regulation leading to impaired cardiovascular function. Additionally alterations in MEF2A function are linked to neurodegenerative disorders such as Alzheimer's disease where it may interact with proteins including Tau and amyloid precursor protein influencing neuronal health and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
This image is courtesy of an anonymous customer reviewWestern blot - Anti-MEF2A (phospho T312) antibody (ab30644)
All lanes: Western blot - Anti-MEF2A (phospho T312) antibody (ab30644) at 1/1000 dilution
All lanes: Mouse liver tissue lysate - whole at 40 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A (phospho T312) antibody (ab30644)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma. Left: using ab30644 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.
Western blot - Anti-MEF2A (phospho T312) antibody (ab30644)
All lanes: Western blot - Anti-MEF2A (phospho T312) antibody (ab30644) at 1 µg/mL
Lane 1: Untreated NIH/3T3 cells (5-30ug).
Lane 2: NIH/3T3 cells treated with PMA (5-30ug)
Predicted band size: 34 kDa, 55 kDa
Observed band size: 65 kDa
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