Anti-MEF2B antibody [EPR22193-26]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on germinal center B cells of human tonsil (PMID : 23974956; PMID : 26089142) (PMID : 26089142) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human diffuse large B-cell lymphoma cells (PMID : 26089142) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on a subset of smooth muscle cells (arrowhead) of human colon (PMID : 26089142; PMID : 8668199) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IP

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Immunoprecipitation - Anti-MEF2B antibody [EPR22193-26] (AB242058)

MEF2B was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab242058 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242058 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Ramos whole lysate 10 μg (Input).
Lane 2 : ab242058 IP in Ramos whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242058 in Ramos whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-MEF2B antibody [EPR22193-26] (ab242058)

Predicted band size: 39 kDa

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• WB

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Western blot - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Blocking and dilution buffer : 5% NFDM/TBST.

The 40-kDa target band is consistent with what has been described in the literature (PMID : 23974956). Minor bands below 40kDa are undetermined proteins, likely to be truncated MEF2B (PMID : 23974956).

Negative control : Hela (PMID : 8798771).

All lanes:

Western blot - Anti-MEF2B antibody [EPR22193-26] (ab242058) at 1/1000 dilution

Lane 1:

Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 2:

Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 3:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 39 kDa

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• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized Daudi (human Burkitt's lymphoma cell line) cells labeling [MEF2B with ab242058 at 1/500 (red) compared with a rabbit monoclonal IgG (ab172730, black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.