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AB309223

Anti-MEF2B antibody [EPR22193-26] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal MEF2B antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

XMEF2, MEF2B, Myocyte-specific enhancer factor 2B, RSRFR2, Serum response factor-like protein 2

1 Images
Sandwich ELISA - Anti-MEF2B antibody [EPR22193-26] - BSA and Azide free (Detector) (AB309223)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MEF2B antibody [EPR22193-26] - BSA and Azide free (Detector) (AB309223)

Sandwich ELISA of ab309263 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22193-26

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEF2B also known as myocyte enhancer factor 2B plays an integral role in regulating gene expression. It is a transcription factor belonging to the MEF2 family which also includes MEF2A MEF2C and MEF2D. MEF2B has a molecular mass of approximately 50 kilodaltons. Researchers have found its expression mainly in lymphoid tissues but it is also present in other tissue types. Through its actions MEF2B influences various cellular processes by binding to DNA and facilitating transcriptional activation.
Biological function summary

MEF2B functions as a regulator of immune system development and differentiation. It acts at the level of transcription to determine the fate of cells by its interactions. MEF2B often partners with various cofactors to form a complex which enhances its ability to control gene expression. It is known to be highly involved in muscle development and cell proliferation underlining its diverse roles in cellular biology.

Pathways

MEF2B plays important roles in two key signaling networks: the MAPK signaling pathway and the calcium signaling pathway. These pathways manage diverse biological processes like cell growth apoptosis and differentiation. Through the MAPK pathway MEF2B interacts with proteins such as ERK leading to various cellular outcomes. In the calcium signaling pathway MEF2B works alongside calcineurin a phosphatase important for activating MEF2 family members.

MEF2B has been linked with specific health conditions particularly in lymphoma and leukemia. Mutations and dysregulation in MEF2B may contribute to the development of these cancers. MEF2B's interaction with other proteins like BCL6 a well-known repressor in lymphomagenesis highlights its importance in these contexts. Understanding MEF2B's role in these diseases may provide insights for potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.
See full target information MEF2B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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