AB250540

Anti-MEF2BNB antibody [EPR14012] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal MEF2BNB antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MEF2BNB, BORCS8, BLOC-1-related complex subunit 8, MEF2B neighbor

1 Images

Supplier Data

Western blot - Anti-MEF2BNB antibody [EPR14012] - BSA and Azide free (AB250540)

This data was developed using ab181872, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MEF2BNB antibody [EPR14012] (<a href='/en-us/products/primary-antibodies/mef2bnb-antibody-epr14012-ab181872'>ab181872</a>) at 1/10000 dilution

Lane 1:

Human small intestine tissue lysate at 10 µg

Lane 2:

Human prostate tissue lysate at 10 µg

Lane 3:

Human skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14012

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250540 is the carrier-free version of ab181872.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEF2BNB also known by the names MEF2-binding nuclear protein is a transcription factor that regulates gene expression. It has a molecular mass of approximately 70 kDa. MEF2BNB is widely expressed in various tissues including cardiac and skeletal muscle where it supports essential cellular functions. MEF2BNB interacts with DNA functioning in the transcriptional regulation of specific genes by modulating enhancer and promoter activities.

Biological function summary

MEF2BNB plays an important role in muscle differentiation and development. MEF2BNB forms a complex with other proteins aiding the assembly of transcriptional machinery at target gene sites. It influences the transcription of genes involved in cellular growth and apoptosis. In muscle cells MEF2BNB adjusts the balance between proliferation and differentiation affecting muscle fiber type specification and adaptation to physical stimuli.

Pathways

MEF2BNB integrates into the MAPK and calcium signaling pathways which regulate various aspects of cellular communication and response to external stimuli. It closely interacts with proteins such as MEF2A and MEF2D in these pathways. MEF2BNB influences gene expression downstream of these signaling cascades integrating signals that dictate cellular responses like growth differentiation and survival.

MEF2BNB shows connections to cardiac diseases and muscular dystrophies. Changes in MEF2BNB expression or function can lead to defective cardiac muscle remodeling contributing to heart failure. Additionally MEF2BNB's interaction with dystrophin links it to muscular dystrophy. Altered MEF2BNB activity can disrupt normal muscle repair and regeneration exacerbating disease conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor.

See full target information BORCS8

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEF2BNB antibody [EPR14012] - BSA and Azide free

Western blot - Anti-MEF2BNB antibody [EPR14012] - BSA and Azide free (AB250540)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise