Name: Anti-MEF2C antibody [EPR19089-202] - ChIP Grade
SKU: ab211493
Rating: 5 (4 reviews)

Rabbit Recombinant Monoclonal MEF2C antibody. Suitable for ChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Images

ChIP - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (AB211493), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ChIP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Tested
Rat	Expected	Tested	Expected	Expected	Tested
Common marmoset	Predicted	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 5 µg chromatin for 25.00000 µg chromatin	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Common marmoset	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Common marmoset	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Common marmoset	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Common marmoset	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Use at 1:500 (human), 1:1000 (mouse, rat) dilution. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Use at 1:500 (human), 1:1000 (mouse, rat) dilution. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Use at 1:500 (human), 1:1000 (mouse, rat) dilution. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Common marmoset	Dilution info -	Notes -

Associated Products

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Target data

See full target information MEF2C

Function

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoforms that lack the repressor domain are more active than isoform 1.

Alternative names

Myocyte-specific enhancer factor 2C, Myocyte enhancer factor 2C, MEF2C

Recommended products

Rabbit Recombinant Monoclonal MEF2C antibody. Suitable for ChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR19089-202
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The MEF2C protein also known as MADS box transcription enhancer factor 2 polypeptide C plays an important role in gene expression regulation. This protein has a mass of approximately 52 kDa and is mainly found in the heart skeletal muscle brain and endothelial cells. It is part of the MEF2 family which includes other members like MEF2A MEF2B and MEF2D. MEF2C acts as a transcription factor binding to specific DNA sequences to activate the transcription of target genes involved in various biological processes.

Biological function summary

The MEF2C protein is essential in cellular differentiation and development. MEF2C is not part of a complex but interacts with other cofactors and regulatory proteins to fulfill its roles. It is important in the development of cardiac and skeletal muscle cells and plays a significant role in neurogenesis and neuronal connectivity in the central nervous system. This protein's precise regulation ensures proper development and function of these tissues and organs.

Pathways

MEF2C influences cellular signaling through the MAPK/ERK and calcium/calmodulin-dependent protein kinase pathways. In the MAPK/ERK pathway MEF2C modulates gene expression in response to external stimuli interacting with proteins like ERK1/2. In the calcium/calmodulin-dependent kinase pathway MEF2C plays a role in calcium signaling networks impacting neuronal plasticity and survival. These pathways highlight MEF2C’s involvement in muscle and brain function.

Associated diseases and disorders

MEF2C mutations or dysregulation link to conditions like dilated cardiomyopathy and neurodevelopmental disorders such as MEF2C haploinsufficiency syndrome. In cardiac disease abnormal MEF2C activity can affect heart muscle cell function often involving proteins like actin and myosin. In neurodevelopmental disorders MEF2C's interaction with various transcriptional regulators impacts neuronal networks contributing to symptoms like impaired cognitive development and autism spectrum-like behaviors.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

ChIP - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Chromatin was prepared from HUVEC cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with: 25 μg of chromatin, 5 μg of ab211493 (red), and 20 μl of protein A/G sepharose beads slurry (10 μl of sepharose A beads + 10 μl of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (SYBR green chemistry).
ChIP was performed according to the literature (PMID: 26923194).
Western blot - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1-2,4: 5 minutes
Lane 3: 15 seconds
Lane 5: 26 seconds
All lanes: Western blot - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493) at 1/1000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Lane 3: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
Lane 4: Mouse brain lysate at 10 µg
Lane 5: Rat brain lysate at 10 µg
Secondary
Lanes 1, 2, 3 and 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Lane 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 50-60 kDa
Flow Cytometry (Intracellular) - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilsed Jurkat (Human T cell leukemia T lymphocyte, Left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cell lines labelling MEF2C with ab211493 at 1/500 dilution (Red) compared with the isotype control Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG Alexa Fluor^® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: Jurkat (PMID: 27876533).
Western blot - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 92 seconds.
The expression profile observed is consistent with the literature (PMID: 18450586). Negative control: Jurkat (PMID: 27876533)
All lanes: Western blot - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493) at 1/1000 dilution
Lane 1: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate 20 μg
Lane 2: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate 20 μg
Lane 3: Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate 20μg
Lane 4: Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 50-60 kDa
Western blot - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 seconds.
All lanes: Western blot - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493) at 1/1000 dilution
Lane 1: Human MEF2C recombinant protein (aa271-493) 10 ng
Lane 2: Human MEF2A recombinant protein (aa1-499, Recombinant Human MEF2A protein (His tag) ab204772) 10 ng
Lane 2: Western blot - Human MEF2C knockout THP-1 cell line (Human MEF2C knockout THP-1 cell line ab288702)
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labelling MEF2C with ab211493 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in B lymphocytes of rat spleen but not in T cells in the periarterial lymphatic sheath is observed (PMID: 8506376, PMID 15703219). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling MEF2C with ab211493 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in B lymphocytes of mouse spleen but not in T cells in the periarterial lymphatic sheath is observed (PMID: 8506376, PMID 15703219). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labelling MEF2C with ab211493 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in leukocytes but not in tumor cells of human endometrial carcinoma is observed (PMID: 8506376, PMID 15703219). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling MEF2C with ab211493 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in human skeletal muscle cells is observed(PMID: 8506376, PMID 15703219). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised Raji (human Burkitt's lymphoma B lymphocyte) cells labelling MEF2C with ab211493 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Confocal image showing nuclear staining in Raji cell line. Negative control: Jurkat (PMID: 27876533). DAPI was used as the nuclear counterstain, and the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 antibody was used as a counterstain at 1/200 dilution.
The negetive controls are as follows:
-ve control 1: Anti-MEF2A + MEF2C antibody [EPR19089-34] ab197070 on jurkat (human T cell leukemia cell line from peripheral blood) cells.
-ve control 2: Jurkat cells stained with DAPI.
-ve control 3: Merged negetive contol images.
Western blot - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493)
False colour image of Western blot: Anti-MEF2C antibody [EPR19089-202] - ChIP Grade staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab211493 was shown to bind specifically to MEF2C. A band was observed at 55/60 kDa in wild-type THP-1 cell lysates with no signal observed at this size in MEF2C knockout cell line (Human MEF2C knockout THP-1 cell line ab313739). To generate this image, wild-type and MEF2C knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-MEF2C antibody [EPR19089-202] - ChIP Grade (ab211493) at 1/1000 dilution
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: MEF2C knockout THP-1 cell lysate at 20 µg
Lane 2: Western blot - Human MEF2C knockout THP-1 cell line (Human MEF2C knockout THP-1 cell line ab313739)
Lane 3: Daudi cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 55/60 kDa, 37 kDa