AB118406

Anti-MEF2C antibody [OTI4B10]

Name: Anti-MEF2C antibody [OTI4B10] (ab118406) | Abcam
Brand: Abcam Limited
SKU: AB118406
Price: 500 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal MEF2C antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MEF2C.

View Alternative Names

Myocyte-specific enhancer factor 2C, Myocyte enhancer factor 2C, MEF2C

4 Images

Flow Cytometry (Intracellular) - Anti-MEF2C antibody [OTI4B10] (AB118406)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MEF2C antibody [OTI4B10] (AB118406)

ab118406 at 1/100 dilution staining MEF2C in HeLa cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MEF2C antibody [OTI4B10] (AB118406)

ab118406 at 1/100 dilution staining MEF2C in Jurkat cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-MEF2C antibody [OTI4B10] (AB118406)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MEF2C antibody [OTI4B10] (AB118406)

ab118406 at 1/100 dilution staining MEF2C in COS7 cells transiently transfected by pCMV6-ENTRY MEF2C by Immunofluorescence.

Supplier Data

Western blot - Anti-MEF2C antibody [OTI4B10] (AB118406)

All lanes:

Western blot - Anti-MEF2C antibody [OTI4B10] (ab118406) at 1/500 dilution

Lane 1:

HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY MEF2C cDNA at 5 µg

Predicted band size: 51 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4B10

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MEF2C.

Q06413

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

The clone number has been updated from 4B10 to OTI4B10, both clone numbers name the same clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MEF2C protein also known as MADS box transcription enhancer factor 2 polypeptide C plays an important role in gene expression regulation. This protein has a mass of approximately 52 kDa and is mainly found in the heart skeletal muscle brain and endothelial cells. It is part of the MEF2 family which includes other members like MEF2A MEF2B and MEF2D. MEF2C acts as a transcription factor binding to specific DNA sequences to activate the transcription of target genes involved in various biological processes.

Biological function summary

The MEF2C protein is essential in cellular differentiation and development. MEF2C is not part of a complex but interacts with other cofactors and regulatory proteins to fulfill its roles. It is important in the development of cardiac and skeletal muscle cells and plays a significant role in neurogenesis and neuronal connectivity in the central nervous system. This protein's precise regulation ensures proper development and function of these tissues and organs.

Pathways

MEF2C influences cellular signaling through the MAPK/ERK and calcium/calmodulin-dependent protein kinase pathways. In the MAPK/ERK pathway MEF2C modulates gene expression in response to external stimuli interacting with proteins like ERK1/2. In the calcium/calmodulin-dependent kinase pathway MEF2C plays a role in calcium signaling networks impacting neuronal plasticity and survival. These pathways highlight MEF2C’s involvement in muscle and brain function.

MEF2C mutations or dysregulation link to conditions like dilated cardiomyopathy and neurodevelopmental disorders such as MEF2C haploinsufficiency syndrome. In cardiac disease abnormal MEF2C activity can affect heart muscle cell function often involving proteins like actin and myosin. In neurodevelopmental disorders MEF2C's interaction with various transcriptional regulators impacts neuronal networks contributing to symptoms like impaired cognitive development and autism spectrum-like behaviors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoforms that lack the repressor domain are more active than isoform 1.

See full target information MEF2C

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 27:271-287 PubMed37122901

2023

Cardiac patches made of brown adipose-derived stem cell sheets and conductive electrospun nanofibers restore infarcted heart for ischemic myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Xinbo Wei,Li Wang,Cuimi Duan,Kai Chen,Xia Li,Ximin Guo,Peng Chen,Haifeng Liu,Yubo Fan

The Journal of investigative dermatology 134:1101-1107 PubMed24304814

2013

miR-223 regulates cell growth and targets proto-oncogenes in mycosis fungoides/cutaneous T-cell lymphoma.

Applications

Unspecified application

Species

Human

Laura Y McGirt,Clare M Adams,Devin A Baerenwald,Jeffrey P Zwerner,John A Zic,Christine M Eischen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEF2C antibody [OTI4B10]

Flow Cytometry (Intracellular) - Anti-MEF2C antibody [OTI4B10] (AB118406)

Flow Cytometry (Intracellular) - Anti-MEF2C antibody [OTI4B10] (AB118406)

Immunocytochemistry/ Immunofluorescence - Anti-MEF2C antibody [OTI4B10] (AB118406)

Western blot - Anti-MEF2C antibody [OTI4B10] (AB118406)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise