AB78888

Anti-MEF2C (phospho S396) antibody

Name: Anti-MEF2C (phospho S396) antibody (ab78888) | Abcam
Brand: Abcam Limited
SKU: AB78888
Price: 535 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(7 Publications)

Rabbit Polyclonal MEF2C phospho S396 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MEF2C pS396.

View Alternative Names

Myocyte-specific enhancer factor 2C, Myocyte enhancer factor 2C, MEF2C

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C (phospho S396) antibody (AB78888)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C (phospho S396) antibody (AB78888)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using ab78888 at 1/50 dilution in the absence or presence of the immunzing phosphopeptide.

Unknown

Western blot - Anti-MEF2C (phospho S396) antibody (AB78888)

All lanes:

Western blot - Anti-MEF2C (phospho S396) antibody (ab78888) at 1/500 dilution

Lane 1:

Extracts from 24 hours starved 3T3 cells at 20 µg

Lane 2:

Extracts from 24 hours starved 3T3 cells at 20 µg with immunizing phosphopeptide

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MEF2C pS396. The exact immunogen used to generate this antibody is proprietary information.

Q06413

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from rabbit antiserum by affinity chromatography, using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MEF2C protein also known as MADS box transcription enhancer factor 2 polypeptide C plays an important role in gene expression regulation. This protein has a mass of approximately 52 kDa and is mainly found in the heart skeletal muscle brain and endothelial cells. It is part of the MEF2 family which includes other members like MEF2A MEF2B and MEF2D. MEF2C acts as a transcription factor binding to specific DNA sequences to activate the transcription of target genes involved in various biological processes.

Biological function summary

The MEF2C protein is essential in cellular differentiation and development. MEF2C is not part of a complex but interacts with other cofactors and regulatory proteins to fulfill its roles. It is important in the development of cardiac and skeletal muscle cells and plays a significant role in neurogenesis and neuronal connectivity in the central nervous system. This protein's precise regulation ensures proper development and function of these tissues and organs.

Pathways

MEF2C influences cellular signaling through the MAPK/ERK and calcium/calmodulin-dependent protein kinase pathways. In the MAPK/ERK pathway MEF2C modulates gene expression in response to external stimuli interacting with proteins like ERK1/2. In the calcium/calmodulin-dependent kinase pathway MEF2C plays a role in calcium signaling networks impacting neuronal plasticity and survival. These pathways highlight MEF2C’s involvement in muscle and brain function.

MEF2C mutations or dysregulation link to conditions like dilated cardiomyopathy and neurodevelopmental disorders such as MEF2C haploinsufficiency syndrome. In cardiac disease abnormal MEF2C activity can affect heart muscle cell function often involving proteins like actin and myosin. In neurodevelopmental disorders MEF2C's interaction with various transcriptional regulators impacts neuronal networks contributing to symptoms like impaired cognitive development and autism spectrum-like behaviors.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoforms that lack the repressor domain are more active than isoform 1.

See full target information MEF2C pS396

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:310 PubMed40000807

2025

Mapk7 enhances osteogenesis and suppresses adipogenesis by activating Lrp6/β-catenin signaling axis in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Li,Jiahui Long,Shuqing Chen,Liru Tian,Ya Xiao,Shulin Chen,Deying Su,Baolin Zhang,Peiqiang Su,Liao Zhiheng,Caixia Xu

Genes & diseases 11:964-977 PubMed37692479

2023

deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling.

Applications

Species

Mouse

Chengzhi Wu,Hengyu Liu,Dongmei Zhong,Xiaoming Yang,Zhiheng Liao,Yuyu Chen,Shun Zhang,Deying Su,Baolin Zhang,Chuan Li,Liru Tian,Caixia Xu,Peiqiang Su

Molecules (Basel, Switzerland) 27: PubMed36014574

2022

MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Xu,Haitong Zhang,Huangting Li,Bo Liu,Rongrong Li,Jinjin Zhang,Xiaodong Song,Changjun Lv,Hongbo Li,Mingwei Chen

Disease models & mechanisms 15: PubMed34553752

2021

MEK-inhibitor-mediated rescue of skeletal myopathy caused by activating Hras mutation in a Costello syndrome mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

William E Tidyman,Alice F Goodwin,Yoshiko Maeda,Ophir D Klein,Katherine A Rauen

Developmental dynamics : an official publication of the American Association of Anatomists 248:795-812 PubMed31219639

2019

Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac outflow tract.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina R Fritz,Yanping Zhang,L Bruno Ruest

Journal of immunology (Baltimore, Md. : 1950) 201:2885-2898 PubMed30305329

2018

B Cell Development sans B Cell Receptor Responsiveness Due to Unfolded Protein Response-Triggered Mef2c Protein Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ting Chen,Yu-Chia Su,John T Kung

Molecular medicine reports 13:2527-35 PubMed26848028

2016

Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells.

Applications

Species

Mouse

Bin Chen,Guixian Song,Ming Liu,Lingmei Qian,Lihua Wang,Haitao Gu,Yahui Shen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEF2C (phospho S396) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C (phospho S396) antibody (AB78888)

Western blot - Anti-MEF2C (phospho S396) antibody (AB78888)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise