Anti-MEF2D antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (AB246884)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MEF2D with ab246884 at a 1/50 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (AB246884)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for MEF2D with ab246884 at a 1/50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (AB246884)

Formalin-fixed, paraffin-embedded human placenta tissue stained for MEF2D with ab246884 at a 1/50 dilution in immunohistochemical analysis.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MEF2D antibody (AB246884)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MEF2D (green) using ab246884 at 4 μg/ml in ICC/IF.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (AB246884)

Formalin-fixed, paraffin-embedded human skeletal liver shows absence of immunoreactivity for MEF2D when incubated with ab246884 at a 1/50 dilution (negative control).

• WB

Unknown

Western blot - Anti-MEF2D antibody (AB246884)

Western blot analysis in human cell line THP-1.

All lanes:

Western blot - Anti-MEF2D antibody (ab246884) at 0.4 µg/mL

All lanes:

THP-1 (human monocytic leukemia cell line) cell lysate

Predicted band size: 56 kDa

false

• WB

Lab

Western blot - Anti-MEF2D antibody (AB246884)

False colour image of Western blot : Anti-MEF2D antibody staining at 0.04 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab246884 was shown to bind specifically to MEF2D. A band was observed at 67 kDa in wild-type HeLa cell lysates with no signal observed at this size in mef2d CRISPR-Cas9 edited cell line ab281636. The band observed in the CRISPR-Cas9 edited lysate lane below 67 kDa is likely to represent a truncated form of MEF2D. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and mef2d CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MEF2D antibody (ab246884) at 0.04 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

mef2d CRISPR-Cas9 edited HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MEF2D knockout HeLa cell line (ab281636)

Lane 3:

U-2 OS cell lysate at 20 µg

Lane 4:

NIH/3T3 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 67 kDa

false

• WB

Supplier Data

Western blot - Anti-MEF2D antibody (AB246884)

All lanes:

Western blot - Anti-MEF2D antibody (ab246884) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 56 kDa

false