Anti-MEF2D antibody [EPR24993-10] (ab282731)

Rabbit Recombinant Monoclonal MEF2D antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB, IHC-P and reacts with Mouse, Human, Rat, Recombinant fragment - Human samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody [EPR24993-10] (AB282731), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	Flow Cyt (Intra)	WB	IHC-P
Human	Tested	Not recommended	Tested	Tested	Not recommended
Mouse	Tested	Not recommended	Tested	Tested	Tested
Rat	Expected	Not recommended	Expected	Tested	Tested
Recombinant fragment - Human	Not recommended	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information MEF2D

Function

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis (By similarity).

Alternative names

Myocyte-specific enhancer factor 2D, MEF2D

Recommended products

Rabbit Recombinant Monoclonal MEF2D antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB, IHC-P and reacts with Mouse, Human, Rat, Recombinant fragment - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24993-10
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MEF2D also known as Myocyte Enhancer Factor 2D is a transcription factor protein with a mass of roughly 55 kDa. Scientists find MEF2D widely expressed in different tissues including muscle brain and immune cells where it regulates gene expression. By binding to specific DNA sequences MEF2D influences the transcription of various genes essential for cellular differentiation and development. MEF2D operates as an important player in controlling the expression of genes involved in muscle development and neuronal differentiation.

Biological function summary

MEF2D plays a significant role in muscle and neuronal development and is part of the MEF2 family which includes other factors such as MEF2A MEF2B and MEF2C. These transcription factors form a complex with other proteins including histone deacetylases (HDACs) to regulate gene expression. MEF2D acts as a mediator between signaling pathways and genes responsible for cell growth survival and apoptosis. It helps modulate cellular responses to various external and internal cues adjusting transcriptional programs accordingly.

Pathways

MEF2D is a significant component in the mitogen-activated protein kinase (MAPK) and calcium/calmodulin-dependent protein kinase (CaMK) signaling pathways. These pathways influence muscle differentiation and neuronal plasticity. MEF2D cooperates with related proteins such as CaMKIV which phosphorylates MEF2D leading to the activation of specific target genes. Its role interconnects with other members of the MEF2 family and influences cellular processes linked to differentiation and survival.

Associated diseases and disorders

MEF2D has been implicated in conditions such as cancer and neurodegenerative diseases. In certain cancers altered regulation of MEF2D expression can lead to unchecked cellular proliferation. Its interaction with HDACs has also been observed in neurodegenerative disorders like Alzheimer's disease where dysregulated gene expression contributes to neuronal dysfunction. MEF2D may interact with other proteins like tau which associates with Alzheimer's pathology making it a target of interest in therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling MEF2D with ab282731 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Nuclear staining on mouse testis (PMID: 24694307). The section was incubated with ab282731 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling MEF2D with ab282731 at 1/50 (11.42 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing mainly nuclear staining in THP-1 cells is observed.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling MEF2D with ab282731 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: SW480/ DLD-1 (PMID: 27364559).
Exposure time: 147 seconds
All lanes: Western blot - Anti-MEF2D antibody [EPR24993-10] (ab282731) at 1/1000 dilution
Lane 1: THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 20 µg
Lane 2: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: DLD-1 (human colon epithelial) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 40 kDa, 70 kDa
Western blot - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Blocking and diluting buffer and concentration: 5% NFDM/TBSTLysates were made freshly and used in WB test immediately to minimize protein degradation.
Exposure time: 147 seconds
All lanes: Western blot - Anti-MEF2D antibody [EPR24993-10] (ab282731) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: 2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling MEF2D with ab282731 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Nuclear staining on rat colon. The section was incubated with ab282731 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling MEF2D with ab282731 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Nuclear staining on mouse cererbum (PMID: 28738418). The section was incubated with ab282731 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling MEF2D with ab282731 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) . Nuclear staining on rat cererbum (PMID: 28738418). The section was incubated with ab282731for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
His-tagged human MEF2D recombinant protein (aa54-328) 10ng
Myc/DDDDK-tagged human MEF2A recombinant protein 10ng
Myc/DDDDK-tagged human MEF2B recombinant protein 10ng
Myc/DDDDK-tagged human MEF2C recombinant protein 10ng
Exposure time: 10 seconds
All lanes: Western blot - Anti-MEF2D antibody [EPR24993-10] (ab282731) at 1/1000 dilution
Lane 1: His-tagged human MEF2D recombinant protein at 0.01 µg
Lane 2: Myc/DDDDK-tagged human MEF2A recombinant protein at 0.01 µg
Lane 3: Myc/DDDDK-tagged human MEF2B recombinant protein at 0.01 µg
Lane 4: Myc/DDDDK-tagged human MEF2C recombinant protein at 0.01 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 31 kDa
Flow Cytometry (Intracellular) - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling MEF2D with ab282731 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEF2D antibody [EPR24993-10] (ab282731)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 cells labelling MEF2D with ab282731 at 1/50 (11.42 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green) Confocal image showing mainly nuclear staining in RAW 264.7 cells is observed.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Western blot - Anti-MEF2D antibody [EPR24993-10] (ab282731)
False colour image of Western blot: Anti-MEF2D antibody [EPR24993-10] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab282731 was shown to bind specifically to MEF2D. A band was observed at 67 kDa in wild-type HeLa cell lysates with no signal observed at this size in mef2d CRISPR-Cas9 edited cell line ab281636. The band observed in the CRISPR-Cas9 edited lysate lane below 67 kDa is likely to represent a truncated form of MEF2D. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and mef2d CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used
Lanes 1 - 4: Western blot - Anti-MEF2D antibody [EPR24993-10] (ab282731) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-MEF2D antibody [EPR24993-10] - BSA and Azide free (Anti-MEF2D antibody [EPR24993-10] - BSA and Azide free ab282733) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Western blot - Human MEF2D knockout HeLa cell line (ab281636)
Lane 2: mef2d CRISPR-Cas9 edited HeLa cell lysate at 20 µg
Lane 3: U-2 OS cell lysate at 20 µg
Lane 4: NIH/3T3 cell lysate at 20 µg
Secondary
All lanes: oat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 67 kDa