AB185961

Anti-Meis homeobox 3/MEIS3 antibody [EPR15416]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Meis homeobox 3/MEIS3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

MRG2, MEIS3, Homeobox protein Meis3, Meis1-related protein 2

4 Images

Flow Cytometry (Intracellular) - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Meis homeobox 3/MEIS3with ab185961 at 1/210 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Detection utilisedGoat anti rabbit IgG (FITC) at 1/150 dilution.

Supplier Data

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

All lanes:

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/5000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

All lanes:

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/5000 dilution

Lane 1:

Human fetal lung tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

All lanes:

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Lane 3:

Rat kidney lysate at 10 µg

Lane 4:

Rat spleen lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15416

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/210", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator which directly modulates PDPK1 expression, thus promoting survival of pancreatic beta-cells. Also regulates expression of NDFIP1, BNIP3, and CCNG1.

See full target information MEIS3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3301 PubMed38671004

2024

Diphthamide deficiency promotes association of eEF2 with p53 to induce p21 expression and neural crest defects.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Shi,Daochao Huang,Cui Song,Ruixue Cao,Zhao Wang,Dan Wang,Li Zhao,Xiaolu Xu,Congyu Lu,Feng Xiong,Haowen Zhao,Shuxiang Li,Quansheng Zhou,Shuyue Luo,Dongjie Hu,Yun Zhang,Cui Wang,Yiping Shen,Weiting Su,Yili Wu,Karl Schmitz,Shuo Wei,Weihong Song

Frontiers in cell and developmental biology 8:556004 PubMed33520978

2021

Tumor-Suppressive Role of microRNA-202-3p in Hepatocellular Carcinoma Through the KDM3A/HOXA1/MEIS3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yijie Zhang,Qi Pan,Zigong Shao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Meis homeobox 3/MEIS3 antibody [EPR15416]

Flow Cytometry (Intracellular) - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (AB185961)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise