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AB250863

Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free

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Rabbit Recombinant Monoclonal Meis homeobox 3/MEIS3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples.

View Alternative Names

MRG2, MEIS3, Homeobox protein Meis3, Meis1-related protein 2

4 Images
Flow Cytometry (Intracellular) - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (AB250863)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (AB250863)

This data was developed using ab185961, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Meis homeobox 3/MEIS3 with ab185961 at 1/210 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Detection utilised Goat anti rabbit IgG (FITC) at 1/150 dilution.

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (AB250863)
  • WB

Supplier Data

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (AB250863)

This data was developed using ab185961, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (<a href='/en-us/products/primary-antibodies/meis-homeobox-3-meis3-antibody-epr15416-ab185961'>ab185961</a>) at 1/5000 dilution

Lane 1:

Human fetal lung tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

false

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (AB250863)
  • WB

Supplier Data

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (AB250863)

This data was developed using ab185961, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (<a href='/en-us/products/primary-antibodies/meis-homeobox-3-meis3-antibody-epr15416-ab185961'>ab185961</a>) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Lane 3:

Rat kidney lysate at 10 µg

Lane 4:

Rat spleen lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (AB250863)
  • WB

Supplier Data

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (AB250863)

This data was developed using ab185961, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (<a href='/en-us/products/primary-antibodies/meis-homeobox-3-meis3-antibody-epr15416-ab185961'>ab185961</a>) at 1/5000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15416

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250863 is the carrier-free version of ab185961.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator which directly modulates PDPK1 expression, thus promoting survival of pancreatic beta-cells. Also regulates expression of NDFIP1, BNIP3, and CCNG1.
See full target information MEIS3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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