AB19867

Anti-MEIS1 antibody

Name: Anti-MEIS1 antibody (ab19867) | Abcam
Brand: Abcam Limited
SKU: AB19867
Price: 605 USD
Availability: InStock
Rating: 4 (3 reviews)

BOND RX™ Validated
What is this?

(3 Reviews)

(42 Publications)

Anti-MEIS1 antibody (ab19867) is a rabbit polyclonal antibody detecting MEIS1 in Western Blot, IHC-P. Suitable for Human, Rat.

- Over 30 publications
- Trusted since 2006

View Alternative Names

Homeobox protein Meis1, MEIS1

3 Images

Project976****

Western blot - Anti-MEIS1 antibody (AB19867)

All lanes:

Western blot - Anti-MEIS1 antibody (ab19867) at 1 µg/mL

All lanes:

HEK293 Wcl Transiently Overexpressing MEIS1 at 5 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

Predicted band size: 43 kDa

Observed band size: 45 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEIS1 antibody (AB19867)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEIS1 antibody (AB19867)

IHC image of MEIS1 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Project

Western blot - Anti-MEIS1 antibody (AB19867)

This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of MEIS1. The predicted molecular weights of isoforms 1 and 2 are 43 kDa and 50 kDa respectively. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-MEIS1 antibody (ab19867) at 1 µg/mL

All lanes:

KG-1 (Human myeloid cell line) Nuclear Lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 43 kDa

Observed band size: 40 kDa,48 kDa,58 kDa

false

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-MEIS1 antibody (ab19867) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Rat samples.

What is the molecular weight of MEIS1?
Anti-MEIS1 (ab19867) specifically detects a band for MEIS1 (UniProt: O00470) at a molecular weight of 43, 50kDa.

Trusted by the scientific community
Anti-MEIS1 (ab19867) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEIS1 known as myeloid ecotropic viral integration site 1 is a homeobox protein that functions as a transcription factor. MEIS1 has a molecular mass of approximately 48 kDa. This protein is expressed in various tissues with high levels noted in the hematopoietic system particularly in progenitor cells. Its role in niave to the human developmental system is well detailed showing influence in developmental processes through gene expression regulation.

Biological function summary

As part of a protein complex MEIS1 interacts with other transcription factors including PBX1 and HOXA9 to modulate target gene expression. MEIS1 plays a critical role in hematopoiesis influencing the development and maintenance of hematopoietic stem and progenitor cells. Also it contributes to limb development and the formation of the heart during embryogenesis. MEIS1’s role in cellular differentiation and organogenesis highlights its significance in developmental biology.

Pathways

MEIS1 acts within the RA (Retinoic Acid) signaling pathway and is also linked to the Wnt signaling pathway. Through these pathways MEIS1 collaborates with other proteins like PBX1 which assist in the regulation of gene expression critical for proper cell fate determination. In the RA pathway MEIS1 modulates the transcription of genes necessary for cell differentiation and proliferation. These pathways demonstrate the broad regulatory influence of MEIS1 on cellular processes.

MEIS1 is linked with an increased risk for restless legs syndrome (RLS) and certain types of leukemia. The interaction of MEIS1 with HOXA9 is notably relevant in the pathogenesis of leukemia as this partnership can cause dysregulation in gene expression leading to oncogenesis. Additionally genetic variations near MEIS1 have been correlated with susceptibility to RLS highlighting its role in neurological conditions. This underlines the clinical importance of MEIS1 in both hematological and neurological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.

See full target information MEIS1

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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40682848

2025

MEIS1‑regulated miR‑488‑3p suppresses the malignant progression of laryngeal squamous cell carcinoma by targeting ACVR1C.

Applications

Unspecified application

Species

Unspecified reactive species

Chunming Zhang,Wenjing Hao,Xinfang Wang,Huina Guo,Long He,Jiao Yang,Ying Wang,Xiwang Zheng,Zhongxun Li,Qi Han,Liqi Wen,Hongliang Liu

The Journal of biological chemistry 301:110320 PubMed40451434

2025

The transcription factor HOXA9 induces expression of the chromatin modifier SMYD3 to drive leukemogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhang,Jinqiu Zhong,Luo Yang,Qianyun Ye,Yanli Jin,Chang Liu,Peilong Lai,Hongle Li,Jingxuan Pan,Bei Jin

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309754 PubMed39162106

2024

Meis1 Targets Protein Tyrosine Phosphatase Receptor J in Fibroblast to Retard Chronic Kidney Disease Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Bai,Shuang Xu,Mingzhu Jiang,Yuxian Guo,Dandan Hu,Jia He,Ting Wang,Yu Zhang,Yan Guo,Yue Zhang,Songming Huang,Zhanjun Jia,Aihua Zhang

iScience 26:107893 PubMed37771659

2023

ZEB2 and MEIS1 independently contribute to hematopoiesis via early hematopoietic enhancer activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yohko Kitagawa,Akihiro Ikenaka,Ryohichi Sugimura,Akira Niwa,Megumu K Saito

Cancer research 83:983-996 PubMed36662812

2023

SWI/SNF Blockade Disrupts PU.1-Directed Enhancer Programs in Normal Hematopoietic Cells and Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Courtney Chambers,Katerina Cermakova,Yuen San Chan,Kristen Kurtz,Katharina Wohlan,Andrew Henry Lewis,Christiana Wang,Anh Pham,Milan Dejmek,Michal Sala,Mario Loeza Cabrera,Rogelio Aguilar,Radim Nencka,H Daniel Lacorazza,Rachel E Rau,H Courtney Hodges

International journal of molecular sciences 23: PubMed36233173

2022

Normal and Aberrant TALE-Class Homeobox Gene Activities in Pro-B-Cells and B-Cell Precursor Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Nagel,Corinna Meyer

Molecular therapy : the journal of the American Society of Gene Therapy 30:3477-3498 PubMed35791879

2022

CircRNA Samd4 induces cardiac repair after myocardial infarction by blocking mitochondria-derived ROS output.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zheng,Senlin Huang,Guoquan Wei,Yili Sun,Chuling Li,Xiaoyun Si,Yijin Chen,Zhenquan Tang,Xinzhong Li,Yanmei Chen,Wangjun Liao,Yulin Liao,Jianping Bin

Journal of advanced research 39:275-289 PubMed35777912

2022

Transcription factor Meis1 act as a new regulator of ischemic arrhythmias in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yining Liu,Jiamin Li,Ning Xu,Hang Yu,Liling Gong,Qingsui Li,Zhenyu Yang,Sijia Li,Jiming Yang,Di Huang,Yadong Xue,Genlong Xue,Jiali Liu,Haixin Chen,Ruijie Zhang,Anqi Li,Yiming Zhao,PengYu Li,Ming Li,Mingbin Liu,Ning Wang,Benzhi Cai

Blood cancer discovery 3:394-409 PubMed35709529

2022

Transcriptional Plasticity Drives Leukemia Immune Escape.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth Eagle,Taku Harada,Jérémie Kalfon,Monika W Perez,Yaser Heshmati,Jazmin Ewers,Jošt Vrabič Koren,Joshua M Dempster,Guillaume Kugener,Vikram R Paralkar,Charles Y Lin,Neekesh V Dharia,Kimberly Stegmaier,Stuart H Orkin,Maxim Pimkin

Nature cancer 3:595-613 PubMed35534777

2022

IKAROS and MENIN coordinate therapeutically actionable leukemogenic gene expression in MLL-r acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon J Aubrey,Jevon A Cutler,Wallace Bourgeois,Katherine A Donovan,Shengqing Gu,Charlie Hatton,Sarah Perlee,Florian Perner,Homa Rahnamoun,Alexandra C P Theall,Jill A Henrich,Qian Zhu,Radosław P Nowak,Young Joon Kim,Salma Parvin,Anjali Cremer,Sarah Naomi Olsen,Nicholas A Eleuteri,Yana Pikman,Gerard M McGeehan,Kimberly Stegmaier,Anthony Letai,Eric S Fischer,X Shirley Liu,Scott A Armstrong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEIS1 antibody

Western blot - Anti-MEIS1 antibody (AB19867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEIS1 antibody (AB19867)

Western blot - Anti-MEIS1 antibody (AB19867)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (42)

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