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Rabbit Recombinant Monoclonal MEIS1 antibody. Suitable for IP, ICC/IF, WB and reacts with Human samples.

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Images

Western blot - Anti-MEIS1 antibody [EPR27231-68] (AB307544), expandable thumbnail
  • Western blot - Anti-MEIS1 antibody [EPR27231-68] (AB307544), expandable thumbnail
  • Immunoprecipitation - Anti-MEIS1 antibody [EPR27231-68] (AB307544), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MEIS1 antibody [EPR27231-68] (AB307544), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.

Alternative names

Homeobox protein Meis1, MEIS1

Recommended products

Rabbit Recombinant Monoclonal MEIS1 antibody. Suitable for IP, ICC/IF, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27231-68
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MEIS1 known as myeloid ecotropic viral integration site 1 is a homeobox protein that functions as a transcription factor. MEIS1 has a molecular mass of approximately 48 kDa. This protein is expressed in various tissues with high levels noted in the hematopoietic system particularly in progenitor cells. Its role in niave to the human developmental system is well detailed showing influence in developmental processes through gene expression regulation.

Biological function summary

As part of a protein complex MEIS1 interacts with other transcription factors including PBX1 and HOXA9 to modulate target gene expression. MEIS1 plays a critical role in hematopoiesis influencing the development and maintenance of hematopoietic stem and progenitor cells. Also it contributes to limb development and the formation of the heart during embryogenesis. MEIS1’s role in cellular differentiation and organogenesis highlights its significance in developmental biology.

Pathways

MEIS1 acts within the RA (Retinoic Acid) signaling pathway and is also linked to the Wnt signaling pathway. Through these pathways MEIS1 collaborates with other proteins like PBX1 which assist in the regulation of gene expression critical for proper cell fate determination. In the RA pathway MEIS1 modulates the transcription of genes necessary for cell differentiation and proliferation. These pathways demonstrate the broad regulatory influence of MEIS1 on cellular processes.

Associated diseases and disorders

MEIS1 is linked with an increased risk for restless legs syndrome (RLS) and certain types of leukemia. The interaction of MEIS1 with HOXA9 is notably relevant in the pathogenesis of leukemia as this partnership can cause dysregulation in gene expression leading to oncogenesis. Additionally genetic variations near MEIS1 have been correlated with susceptibility to RLS highlighting its role in neurological conditions. This underlines the clinical importance of MEIS1 in both hematological and neurological disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-MEIS1 antibody [EPR27231-68] (ab307544), expandable thumbnail

    Western blot - Anti-MEIS1 antibody [EPR27231-68] (ab307544)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human MEIS2 and MEIS3.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-MEIS1 antibody [EPR27231-68] (ab307544) at 1/1000 dilution

    Lane 1: HEK-293T cells transfected with a human MEIS1 expression vector containing a his tag whole cell lysate at 40 µg

    Lane 2: HEK-293T cells transfected with a human MEIS2 expression vector containing a his tag whole cell lysate at 40 µg

    Lane 3: HEK-293T cells transfected with a human MEIS3 expression vector containing a his tag whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 51 kDa

    Exposure time: 15s

  • Western blot - Anti-MEIS1 antibody [EPR27231-68] (ab307544), expandable thumbnail

    Western blot - Anti-MEIS1 antibody [EPR27231-68] (ab307544)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Daudi (PMID: 11094311)

    Exposure time: 15 seconds.

    All lanes: Western blot - Anti-MEIS1 antibody [EPR27231-68] (ab307544) at 1/1000 dilution

    Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 2: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot at 1/2000 dilution

    Observed band size: 51 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-MEIS1 antibody [EPR27231-68] (ab307544), expandable thumbnail

    Immunoprecipitation - Anti-MEIS1 antibody [EPR27231-68] (ab307544)

    MEIS1 was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 µg with ab307544 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307544 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: IMR-32 whole cell lysate 10 µg

    Lane 2: ab307544 IP in IMR-32 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307544 in IMR-32 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5.5 seconds .

    All lanes: Immunoprecipitation - Anti-MEIS1 antibody [EPR27231-68] (ab307544) at 1/30 dilution

    All lanes: IMR-32 (human neuroblastoma neuroblast)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 5.5s

  • Immunocytochemistry/ Immunofluorescence - Anti-MEIS1 antibody [EPR27231-68] (ab307544), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MEIS1 antibody [EPR27231-68] (ab307544)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized IMR-32 (human neuroblastoma neuroblast) cells labellng MEIS1 with ab307544 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
    Confocal image showing nuclear staining in IMR-32 cell line.
    Negative control: Daudi (PMID: 11094311).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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