Anti-MEIS1 antibody [EPR27231-68] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MEIS1 antibody [EPR27231-68] - BSA and Azide free (AB307545)

This data was developed using ab307544, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized IMR-32 (human neuroblastoma neuroblast) cells labellng MEIS1 with ab307544 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in IMR-32 cell line. Negative control : Daudi (PMID : 11094311). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-MEIS1 antibody [EPR27231-68] - BSA and Azide free (AB307545)

This data was developed using ab307544, the same antibody clone in a different buffer formulation. MEIS1 was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 µg with ab307544 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307544 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : IMR-32 whole cell lysate 10 µg Lane 2 : ab307544 IP in IMR-32 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307544 in IMR-32 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5.5 seconds .

All lanes:

Immunoprecipitation - Anti-MEIS1 antibody [EPR27231-68] (<a href='/en-us/products/primary-antibodies/meis1-antibody-epr27231-68-ab307544'>ab307544</a>) at 1/30 dilution

All lanes:

IMR-32 (human neuroblastoma neuroblast)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 5.5s

• WB

Supplier Data

Western blot - Anti-MEIS1 antibody [EPR27231-68] - BSA and Azide free (AB307545)

This data was developed using ab307544, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : Daudi (PMID : 11094311) Exposure time : 15 seconds.

All lanes:

Western blot - Anti-MEIS1 antibody [EPR27231-68] (<a href='/en-us/products/primary-antibodies/meis1-antibody-epr27231-68-ab307544'>ab307544</a>) at 1/1000 dilution

Lane 1:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 40 µg

Lane 2:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 51 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-MEIS1 antibody [EPR27231-68] - BSA and Azide free (AB307545)

This data was developed using ab307544, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human MEIS2 and MEIS3.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-MEIS1 antibody [EPR27231-68] (<a href='/en-us/products/primary-antibodies/meis1-antibody-epr27231-68-ab307544'>ab307544</a>) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a human MEIS1 expression vector containing a his tag whole cell lysate at 40 µg

Lane 2:

HEK-293T cells transfected with a human MEIS2 expression vector containing a his tag whole cell lysate at 40 µg

Lane 3:

HEK-293T cells transfected with a human MEIS3 expression vector containing a his tag whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 51 kDa

true

Exposure time: 15s