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Rabbit Recombinant Monoclonal MEIS2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-MEIS2 antibody [EPR12100] - BSA and Azide free (AB249781), expandable thumbnail
  • Western blot - Anti-MEIS2 antibody [EPR12100] - BSA and Azide free (AB249781), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Rat
Not recommended
Predicted
Not recommended
Not recommended
Predicted

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Involved in transcriptional regulation. Binds to HOX or PBX proteins to form dimers, or to a DNA-bound dimer of PBX and HOX proteins and thought to have a role in stabilization of the homeoprotein-DNA complex. Isoform 3 is required for the activity of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element; MEIS2 is not involved in complex DNA-binding. Probably in complex with PBX1, is involved in transcriptional regulation by KLF4. Isoform 3 and isoform 4 can bind to a EPHA8 promoter sequence containing the DNA motif 5'-CGGTCA-3'; in cooperation with a PBX protein (such as PBX2) is proposed to be involved in the transcriptional activation of EPHA8 in the developing midbrain. May be involved in regulation of myeloid differentiation. Can bind to the DNA sequence 5'-TGACAG-3'in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription; isoform 5 cannot activate DRD1 transcription.

Alternative names

Homeobox protein Meis2, Meis1-related protein 1, MEIS2, MRG1

Recommended products

Rabbit Recombinant Monoclonal MEIS2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR12100

Purification technique

Affinity purification

Specificity

The immunogen of this antibody is, according to BLAST results, highly conserved (93%) with human MEIS1. According to this, the antibody is very likely to cross-react with MEIS1 but this has, to our knowledge, not been experimentally tested so far.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab249781 is the carrier-free version of Anti-MEIS2 antibody [EPR12100] ab174270.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MEIS2 or Myeloid Ecotropic Viral Integration Site 2 is a transcription factor known for its role in gene regulation. This protein weighs approximately 52-54 kDa and participates in DNA binding. MEIS2 is highly expressed in tissues like the brain heart and kidneys especially during developmental stages. MEIS2 functions through direct interaction with genomic regions to regulate the transcription of target genes.

Biological function summary

MEIS2 influences cell differentiation and organ development. It interacts with other proteins as part of a complex including the HOX and PBX families to fine-tune transcriptional outcomes. MEIS2 plays a role in neuronal development where its activity helps shape the neural tissue architecture. Researchers observe that MEIS2 affects cellular processes important for forming correctly structured organs.

Pathways

MEIS2 is engaged in the retinoic acid signaling pathway and the Notch signaling pathway. These pathways are vital in embryonic development and cellular differentiation. MEIS2's interaction with the PBX1 protein demonstrates its importance in pathways that lead to precise gene expression during development. Together they contribute to regulating the transcriptional networks involved in developmental processes.

Associated diseases and disorders

MEIS2 has links to certain developmental disorders and cancers. Mutations or aberrant expression of MEIS2 correlate with conditions like cleft palate and neuroblastoma. Notably its interaction with the HOXA9 protein has been documented in certain types of leukemia showing how MEIS2-related pathways can contribute to abnormal cell proliferation. Understanding these interactions aids research into potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry (Intracellular) - Anti-MEIS2 antibody [EPR12100] - BSA and Azide free (ab249781), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MEIS2 antibody [EPR12100] - BSA and Azide free (ab249781)

    This data was developed using Anti-MEIS2 antibody [EPR12100] ab174270, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of permeabilized K562 cells using Anti-MEIS2 antibody [EPR12100] ab174270 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-MEIS2 antibody [EPR12100] - BSA and Azide free (ab249781), expandable thumbnail

    Western blot - Anti-MEIS2 antibody [EPR12100] - BSA and Azide free (ab249781)

    This data was developed using Anti-MEIS2 antibody [EPR12100] ab174270, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-MEIS2 antibody [EPR12100] (Anti-MEIS2 antibody [EPR12100] ab174270) at 1/1000 dilution

    Lane 1: Fetal thymus lysates at 10 µg

    Lane 2: HeLa lysates at 10 µg

    Lane 3: HepG2 lysates at 10 µg

    Lane 4: K562 lysates at 10 µg

    Predicted band size: 52 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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