AB278716

Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]

20ul selling size
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal MEK3 phospho S189 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human, African green monkey samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAP2K3 phospho S189.

View Alternative Names

MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2, MEK6, MKK6, PRKMK6, SKK3, MAP2K6, Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6, MAPKK 6, MAPK/ERK kinase 6, Stress-activated protein kinase kinase 3, MEK 6, SAPK kinase 3, SAPKK-3, SAPKK3

4 Images

Flow Cytometry - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

Peptide blocking flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using ab278716 at 0.1 μg/ml.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells secondary antibody only negative control (blue) or treated with K252a (red) or treated with UV+TPA (green) using ab278716 at 0.5 μg/ml.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells treated with secondary antibody only (blue) or 0.1 μg/ml of isotype control (orange) or ab278716.

Supplier Data

Western blot - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

All lanes:

Western blot - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (ab278716) at 0.05 µg/mL

Lane 1:

Untreated COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts

Lane 2:

UV-treated COS-7 cell extracts

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578 PE

PE Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]
660 APC

APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]
519 FITC

FITC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MKK3S189MKK6S207-D3

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Mouse, African green monkey, Human

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human MAP2K3 phospho S189. The exact immunogen used to generate this antibody is proprietary information.

P46734

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK3 also known as MAP2K3 and MKK6 known as MAP2K6 are protein kinases involved in the MAP kinase signaling pathway. MEK3 has a molecular weight of approximately 38 kDa and MEK6 has a similar molecular weight. Researchers have identified these kinases in various human tissues with significant expression in muscles heart and brain tissues. Both MEK3 and MKK6 activate specific downstream MAP kinases via phosphorylation facilitating their role in cellular stress responses.

Biological function summary

MEK3 and MKK6 mediate responses to stress and inflammatory cytokines marking their importance in signal transduction. They activate p38 MAP kinase through phosphorylation a part of a complex necessary for cellular responses. This activity influences gene expression related to cellular growth differentiation and apoptosis. MEK3 and MKK6 show functional overlap and redundancy ensuring robust cellular responses to external stimuli.

Pathways

MEK3 and MKK6 are integral in the MAP kinase signaling pathway and the p38 MAPK pathway. These pathways play critical roles in cell differentiation apoptosis and response to extracellular stress signals. MEK3 and MEK6 phosphorylate and activate the p38 MAPK protein which regulates stress-induced gene expression. This relationship emphasizes the interplay between kinases and how MEK3 and MEK6 fit within broader signaling networks.

MEK3 and MKK6 are associated with inflammatory diseases and cancer. Dysregulation of the p38 MAPK pathway involving MEK3 and MKK6 contributes to these conditions due to improper stress response signaling. Notably MEK6's interaction with the p38 protein highlights its potential role in exacerbating inflammation and tumorigenesis. Understanding the mechanisms involving MEK3 and MKK6 assists in the development of targeted therapies for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

See full target information MAP2K3 phospho S189

Additional targets

MAP2K6 phospho S207

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 19:1144-1163 PubMed36170234

2022

FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Cai,Mengyan Hu,Chunyi Li,Ruizhen Wu,Danli Lu,Chichu Xie,Wei Zhang,Tiemei Li,Shishi Shen,Huipeng Huang,Wei Qiu,Quentin Liu,Yan Lu,Zhengqi Lu

Balkan medical journal 39:267-274 PubMed35872627

2022

FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Shulan Dou

Cell cycle (Georgetown, Tex.) 21:741-753 PubMed35258413

2022

Hsa-miR-186-3p suppresses colon cancer progression by inhibiting KRT18/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Xia,Zhiyong Zhang,Xin Zhang,Quanfu Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]

Flow Cytometry - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

Flow Cytometry - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

Flow Cytometry - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

Western blot - Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278716)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

Product promise