AB194754

Anti-MEK1 + MEK2 (phospho S218 + S222) antibody

Name: Anti-MEK1 + MEK2 (phospho S218 + S222) antibody (ab194754) | Abcam
Brand: Abcam Limited
SKU: AB194754
Price: 535 USD
Availability: InStock

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(34 Publications)

Rabbit Polyclonal MEK2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 34 publications. Immunogen corresponding to Synthetic Peptide within Human MAP2K1 phospho S218 + S222.

View Alternative Names

MEK2, MKK2, PRKMK2, MAP2K2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2

1 Images

Supplier Data

Western blot - Anti-MEK1 + MEK2 (phospho S218 + S222) antibody (AB194754)

All lanes:

Western blot - Anti-MEK1 + MEK2 (phospho S218 + S222) antibody (ab194754) at 1/500 dilution

Lane 1:

HeLa cell extract, treated with UV

Lane 2:

HeLa cell extract, untreated

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MAP2K1 phospho S218 + S222. The exact immunogen used to generate this antibody is proprietary information.

Q02750

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK1 and MEK2 also known as MAP2K1 and MAP2K2 respectively are dual-specificity kinases that play roles in signaling pathways mediating cellular responses. MEK1 and MEK2 have molecular weights of approximately 45kDa. They are expressed in various tissues including the heart brain and skeletal muscles reflecting their widespread function within the organism. These proteins are often referred collectively as the MEK1/2 complex due to their shared and overlapping roles in cell signaling.

Biological function summary

MEK1 and MEK2 are integral parts of the mitogen-activated protein kinase (MAPK) signaling cascade. MEK1/2 phosphorylate and activate the ERK1/2 kinases which transmit signals from the cell surface to the nucleus regulating gene expression and cell fate decisions. The MEK1/2 proteins also act in concert with other kinases and substrates forming signaling complexes that ensure specific and regulated cellular outcomes.

Pathways

MEK1 and MEK2 play key roles within the MAPK/ERK pathway a critical signaling mechanism for cell division and survival. They also link to the RAS/MAPK pathway involved in transmitting signals from membrane receptors to the nucleus. In these pathways MEK1/2 interact with proteins like RAF kinases and ERK1/2 propagating signals that influence cellular growth and differentiation.

MEK1 and MEK2 are implicated in cancer and neurological disorders. Abnormal activity of the MEK1/2 proteins often due to mutations can lead to uncontrolled cell proliferation contributing to the development of cancers such as melanoma. In neurological disorders dysregulation of MEK1/2 signaling relates to conditions like neuro-cardio-facial-cutaneous syndromes. These proteins interact with other key players in these diseases such as mutant RAF proteins in melanoma highlighting their critical roles in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed : 29433126).

See full target information MAP2K2

Additional targets

MAP2K2

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Cancer innovation 3:e117 PubMed38947754

2024

MAPK4 facilitates angiogenesis by inhibiting the ERK pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Jing Yang,Yufang Liu,Xu Zhao,Juanjuan Zhao,Lin Tang,Mengmeng Guo,Ya Zhou,Chao Chen,Dongmei Li,Zhenke Wen,Guiyou Liang,Lin Xu

Microbiology spectrum 12:e0283823 PubMed38197658

2024

Application of bacteriophage φPaP11-13 attenuates rat infection lesions by promoting keratinocytes apoptosis via inhibiting PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Liu,Ni Zhen,Danxi Liao,Jiahui Niu,Ruolan Liu,Zijiao Li,Zeyuan Lei,Zichen Yang

Cell biochemistry and function 41:857-867 PubMed37606071

2023

Xiebai Zengye decoction improves respiratory function and attenuates inflammation in juvenile rats with postinfection cough via regulating ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Luo,Yijue Deng,Yi Ding,Chenguang Tang,Mengqing Wang

Cellular and molecular bioengineering 16:159-171 PubMed37096071

2023

Circ_0004585 Facilitates Tumorigenesis of Colorectal Cancer Modulating the miR-338-3p/ZFX Axis and Activating the MEK/ERK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zenghai Lin,Jianwei Lin

Histology and histopathology 38:1381-1390 PubMed36537752

2022

Transcription factor ZNF488 accelerates cervical cancer progression through regulating the MEK/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kena Weng,Lu Li,Haiping Zhou

International journal of molecular sciences 23: PubMed36361610

2022

Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs.

Applications

Unspecified application

Species

Unspecified reactive species

Yuh-Fung Chen,Kuo-Jen Wu,Lian-Ru Siao,Huei-Yann Tsai

International heart journal 62:1124-1134 PubMed34497168

2021

LncRNA RP11-400K9.4 Aggravates Cardiomyocytes Apoptosis After Hypoxia/Reperfusion Injury by Targeting miR-423.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiong Zhang,Chao Yang,Binghua Qiu

Cell death discovery 7:177 PubMed34238921

2021

Implication of human endogenous retrovirus W family envelope in hepatocellular carcinoma promotes MEK/ERK-mediated metastatic invasiveness and doxorubicin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhou,Lijuan Liu,Youyi Liu,Ping Zhou,Qiujin Yan,Honglian Yu,Xiaobei Chen,Fan Zhu

Antioxidants (Basel, Switzerland) 10: PubMed34208998

2021

MEK1/2-ERK Pathway Alterations as a Therapeutic Target in Sporadic Alzheimer's Disease: A Study in Senescence-Accelerated OXYS Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia A Muraleva,Nataliya G Kolosova,Natalia A Stefanova

Analytical cellular pathology (Amsterdam) 2021:6668947 PubMed34239803

2021

Activation of MEK1/2/Nrf-2 Signaling Pathway by Epstein-Barr Virus-Latent Membrane Protein 1 Enhances Autophagy and Cisplatin Resistance in T-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xintao Jia,Qiuyu He,Mei Zeng,Yuhua Chen,Yan Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MEK1 + MEK2 (phospho S218 + S222) antibody

Western blot - Anti-MEK1 + MEK2 (phospho S218 + S222) antibody (AB194754)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (34)

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