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AB278564

Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3]

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(12 Publications)

Rabbit Recombinant Monoclonal MEK1 phospho S221 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human MAP2K1 phospho S221.

View Alternative Names

MEK1, PRKMK1, MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1

6 Images
Flow Cytometry - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278564 at 0.1 µg/mL.

Flow Cytometry - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate (green) or imatinib and blocked with phospho peptide (gold) or pervanadate and blocked with phospho peptide (black) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278564 at 0.1 μg/mL.

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)
  • WB

Supplier Data

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)

All lanes:

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564) at 0.1 µg/mL

Lane 1:

HT1080 cell extract treated with TPA

Lane 2:

HT1080 cell extract untreated with TPA

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Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)
  • WB

Supplier Data

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)

Western blot analysis of C2C12 cell extract untreated or treated with TPA using 0.1 µg/mL ab278564.

All lanes:

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

false

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)
  • WB

Supplier Data

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)

All lanes:

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564) at 0.1 µg/mL

Lane 1:

A549 cell extract treated with TPA

Lane 2:

A549 cell extract untreated with TPA

false

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)
  • WB

Supplier Data

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (AB278564)

All lanes:

Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564) at 0.1 µg/mL

Lane 1:

NIH3T3 cell extract untreated with TPA

Lane 2:

NIH3T3 cell extract treated with TPA

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MEK12S221-D3

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human MAP2K1 phospho S221. The exact immunogen used to generate this antibody is proprietary information.

Q02750

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK1 and MEK2 also known as MAP2K1 and MAP2K2 are protein kinases with molecular weights approximately 43 kDa and 45 kDa respectively. These proteins belong to the MAPKK family and function as dual-specificity kinases phosphorylating both serine/threonine and tyrosine residues. MEK1 and MEK2 are expressed ubiquitously in human tissues indicating their widespread role in cellular processes. Their main mechanical role is to activate ERK1/2 which is important in various signal transduction pathways. As part of the MAPK/ERK cascade MEK proteins serve as a conduit for signals from the membrane to the nucleus.
Biological function summary

MEK1 and MEK2 participate in transmitting signals that regulate cell proliferation differentiation and survival. Their role in these processes involves forming part of a signaling complex that includes RAF kinases and ERK1/2. This assembly ensures precise regulation of cell cycle events and growth responses to extracellular signals. The activity of MEK proteins allows cells to modulate responses to external growth factors and stress maintaining cellular homoeostasis. Given their involvement in key biological processes MEK1 and MEK2 are essential for normal cellular function.

Pathways

MEK1 and MEK2 play significant roles in the MAPK/ERK and PI3K/Akt pathways. Their activation by upstream kinases like RAF enables downstream phosphorylation and activation of ERK1/2 key regulators of gene expression and cellular activities. The MAPK/ERK pathway interconnects with other pathways such as the PI3K/Akt pathway creating a network of signals important for cell fate decisions. Through these pathways MEK1 and MEK2 interact with a variety of proteins including PI3K and Akt linking growth factor signaling to broader cellular outcomes.

MEK1 and MEK2 exhibit distinct connections to cancers and RASopathies. Aberrant activation of the MAPK/ERK pathway often through mutations in MEK1/2 contributes to oncogenesis in several cancer types including melanoma and colorectal cancer. Additionally changes in these proteins are implicated in RASopathies such as Noonan syndrome a series of genetic disorders caused by dysregulation of the RAS-MAPK pathway. In these conditions MEK proteins often show altered interactions with other proteins like RAF and RAS correlating with disease pathogenesis and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed : 29433126). Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
See full target information MAP2K1 phospho S221

Additional targets

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:105264 PubMed40697228

2025

Nav1.6 drives colorectal cancer proliferation and invasion through MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ming Zhao,Wan-Ying Hong,Jian-Guang Xu,Shui-Quan Lin,Ming-Sheng Liu,Li-Hui Wang,Xu-Li Jiang,Ming Sang,Yang-Bo Lv

Journal of cell communication and signaling 19:e70025 PubMed40520075

2025

Adipose-derived stem cell exosomes alleviate TGF-β1-induced urethral stricture fibrosis by suppressing the TGF-β/Smad pathway and downstream PDGFR-β/RAS/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liang,Chao Deng,Hang Guo,Zhenghao Dai,Yiwen Jiang,Yuting Lu,Weiguo Chen

BMC cancer 24:650 PubMed38802739

2024

CD276 enhances sunitinib resistance in clear cell renal cell carcinoma by promoting DNA damage repair and activation of FAK-MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Yu Zhang,Jian-Hao Xu,Jiang-Lei Zhang,Yu-Xin Lin,Jun Ou-Yang

Acta pharmaceutica (Zagreb, Croatia) 73:269-280 PubMed37307376

2023

Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma .

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Cuihong Jiang,Zhizheng Zhao,Yutong Lv,Gaoxing Wang

Nature communications 13:5945 PubMed36209205

2022

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan Xu

Disease markers 2022:7594489 PubMed35903293

2022

COPS3 Promotes Proliferation, Invasion, and EMT of Colorectal Cancer Cells by MEK/ERK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchao Xie,Zhijiang Wei,Chi Cheng

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer 41:89-98 PubMed35695654

2022

Ginsenoside Rk2 Protects against Ulcerative Colitis via Inactivating ERK/MEK Pathway by SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Huang,Jianwei Xiao,Mudan Wen,Jinghua Liang

Journal of immunology research 2022:3351268 PubMed35571569

2022

Echinacoside Induces Mitochondria-Mediated Pyroptosis through Raf/MEK/ERK Signaling in Non-Small Cell Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Shi,Hui Cao,Zhengcheng Liu,Lei Xi,Changqing Dong

Pharmaceutical biology 60:764-773 PubMed35387566

2022

Caudatin blocks the proliferation, stemness and glycolysis of non-small cell lung cancer cells through the Raf/MEK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Hou,Qing Chen,Yufeng Huang,Zhiwei Wu,De Ma

Journal of Cancer 13:1972-1984 PubMed35399719

2022

METTL3 Facilitates Tumor Progression by COL12A1/MAPK Signaling Pathway in Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Li,Zhenhua Li,Yanzhao Xu,Chao Huang,Baoen Shan
View all publications
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