Anti-MEK1 (phospho S298) antibody [MEK1S298-H8]
- 20ul selling size
- Recombinant
- What is this?
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(1 Publication)
Rabbit Recombinant Monoclonal MEK1 phospho S298 antibody. Suitable for Flow Cyt, WB and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAP2K1 phospho S298.
View Alternative Names
MEK1, PRKMK1, MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)
Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green) using ab278562 at 0.5 μg/mL.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)
Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with MG132 (green) or treated and blocked with phospho peptide (orange) or treated and blocked with non-phospho peptide (purple) using ab278562 at 0.1 µg/mL.
- WB
Supplier Data
Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)
All lanes:
Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562) at 0.01 µg/mL
Lane 1:
C6 cell extract treated with TPA
Lane 2:
C6 cell extract untreated with TPA
Predicted band size: 43 kDa
false
- WB
Supplier Data
Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)
All lanes:
Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562) at 0.005 µg/mL
Lane 1:
C6 cell extract treated with TPA
Lane 2:
C6 cell extract untreated with TPA
Predicted band size: 43 kDa
false
Reactivity data
Product details
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MEK1 impacts cell processes such as proliferation differentiation and survival. It does not work alone but forms a complex with other proteins to exert its function. MEK1 activity is tightly controlled by upstream activators and downstream targets. One of the key phosphorylation sites on MEK1 is at serine 298 often marked as MEK1 pS298. This phosphorylation indicates MEK1's activated state which is critical for its biological function.
Pathways
MEK1 integrates signals within the MAPK/ERK pathway and interacts closely with RAF kinases upstream and ERK kinases downstream. This pathway modulates cellular responses to growth factors. MEK1 undergoes phosphorylation enhancing its activity and subsequently phosphorylates ERK proteins. Many studies isolate MEK1 activity using specific inhibitors such as the MEK1 inhibitor to dissect pathway dynamics. ERK inhibitor PD98059 also helps when studying parallel interactions with MEK1.
Product protocols
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Target data
Publications (1)
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Aging 13:16786-16803 PubMed34162761
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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