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AB278562

Anti-MEK1 (phospho S298) antibody [MEK1S298-H8]

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(1 Publication)

Rabbit Recombinant Monoclonal MEK1 phospho S298 antibody. Suitable for Flow Cyt, WB and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAP2K1 phospho S298.

View Alternative Names

MEK1, PRKMK1, MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1

4 Images
Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)

Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green) using ab278562 at 0.5 μg/mL.

Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with MG132 (green) or treated and blocked with phospho peptide (orange) or treated and blocked with non-phospho peptide (purple) using ab278562 at 0.1 µg/mL.

Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)
  • WB

Supplier Data

Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)

All lanes:

Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562) at 0.01 µg/mL

Lane 1:

C6 cell extract treated with TPA

Lane 2:

C6 cell extract untreated with TPA

Predicted band size: 43 kDa

false

Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)
  • WB

Supplier Data

Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (AB278562)

All lanes:

Western blot - Anti-MEK1 (phospho S298) antibody [MEK1S298-H8] (ab278562) at 0.005 µg/mL

Lane 1:

C6 cell extract treated with TPA

Lane 2:

C6 cell extract untreated with TPA

Predicted band size: 43 kDa

false

  • 519 FITC

    FITC Anti-MEK1 (phospho S298) antibody [MEK1S298-H8]

  • 578 PE

    PE Anti-MEK1 (phospho S298) antibody [MEK1S298-H8]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MEK1S298-H8

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Rat, Human

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human MAP2K1 phospho S298. The exact immunogen used to generate this antibody is proprietary information.

Q02750

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.005-0.01 µg/mL", "WB-species-notes": "<p></p>" } } }

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK1 also known as MAP2K1 is a dual-specificity kinase that plays a role in the MAPK/ERK pathway. It has a molecular weight of around 45 kDa. MEK1 phosphorylates and activates the extracellular signal-regulated kinases ERK1 and ERK2. MEK1 is expressed in various tissues but is especially abundant in brain and heart tissues. Researchers commonly measure MEK1 levels using techniques like MEK1 ELISA while phospho-MEK antibodies help analyze its activity state.
Biological function summary

MEK1 impacts cell processes such as proliferation differentiation and survival. It does not work alone but forms a complex with other proteins to exert its function. MEK1 activity is tightly controlled by upstream activators and downstream targets. One of the key phosphorylation sites on MEK1 is at serine 298 often marked as MEK1 pS298. This phosphorylation indicates MEK1's activated state which is critical for its biological function.

Pathways

MEK1 integrates signals within the MAPK/ERK pathway and interacts closely with RAF kinases upstream and ERK kinases downstream. This pathway modulates cellular responses to growth factors. MEK1 undergoes phosphorylation enhancing its activity and subsequently phosphorylates ERK proteins. Many studies isolate MEK1 activity using specific inhibitors such as the MEK1 inhibitor to dissect pathway dynamics. ERK inhibitor PD98059 also helps when studying parallel interactions with MEK1.

MEK1 mutations or dysregulation have links to several types of cancer including melanoma. These often involve changes that lead to continuous MEK1 activation contributing to uncontrolled cell growth. MEK1 involvement in the RASopathies also shows its connection to disorders characterized by heart and facial abnormalities. In cancer MEK1 works closely with the BRAF protein which often harbors mutations leading to disease progression showcasing MEK1 as a target for therapeutic intervention.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed : 29433126). Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
See full target information MAP2K1 phospho S298

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 13:16786-16803 PubMed34162761

2021

Transcription factor PAX4 facilitates gastric cancer progression through interacting with miR-27b-3p/Grb2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Li Ding,Qingfeng Ni,Ran Tao,Jun Qin
View all publications

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