Knockout Tested Rabbit Recombinant Monoclonal MEK1 phospho T286 antibody. Carrier free. Suitable for WB, ICC/IF, Dot and reacts with Human, Mouse, Synthetic peptide, Synthetic peptide - Mouse samples.
Images
![Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--western-blot-img408826.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510)")
![Dot Blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--dot-blot-img408880.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510)")
![Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--immunocytochemistry-immunofluorescence-img408879.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510)")
![Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--immunocytochemistry-immunofluorescence-img408878.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510)")
![Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--western-blot-img408827.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (AB307510)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Dot | IHC-P | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Synthetic peptide - Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Synthetic peptide - Mouse, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Synthetic peptide - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide, Synthetic peptide - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide, Synthetic peptide - Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide, Synthetic peptide - Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (By similarity). Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Alternative names
Mek1, Prkmk1, Map2k1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal MEK1 phospho T286 antibody. Carrier free. Suitable for WB, ICC/IF, Dot and reacts with Human, Mouse, Synthetic peptide, Synthetic peptide - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27226-8
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEK1 also known as MAP2K1 is a dual-specificity kinase that plays a role in the MAPK/ERK pathway. It has a molecular weight of around 45 kDa. MEK1 phosphorylates and activates the extracellular signal-regulated kinases ERK1 and ERK2. MEK1 is expressed in various tissues but is especially abundant in brain and heart tissues. Researchers commonly measure MEK1 levels using techniques like MEK1 ELISA while phospho-MEK antibodies help analyze its activity state.
- Biological function summary
MEK1 impacts cell processes such as proliferation differentiation and survival. It does not work alone but forms a complex with other proteins to exert its function. MEK1 activity is tightly controlled by upstream activators and downstream targets. One of the key phosphorylation sites on MEK1 is at serine 298 often marked as MEK1 pS298. This phosphorylation indicates MEK1's activated state which is critical for its biological function.
- Pathways
MEK1 integrates signals within the MAPK/ERK pathway and interacts closely with RAF kinases upstream and ERK kinases downstream. This pathway modulates cellular responses to growth factors. MEK1 undergoes phosphorylation enhancing its activity and subsequently phosphorylates ERK proteins. Many studies isolate MEK1 activity using specific inhibitors such as the MEK1 inhibitor to dissect pathway dynamics. ERK inhibitor PD98059 also helps when studying parallel interactions with MEK1.
- Associated diseases and disorders
MEK1 mutations or dysregulation have links to several types of cancer including melanoma. These often involve changes that lead to continuous MEK1 activation contributing to uncontrolled cell growth. MEK1 involvement in the RASopathies also shows its connection to disorders characterized by heart and facial abnormalities. In cancer MEK1 works closely with the BRAF protein which often harbors mutations leading to disease progression showcasing MEK1 as a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--western-blot-img408826.jpg "Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)")
Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)
This data was produced using Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509, the same clone but in a different formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
In Western blot, anti-MEK1+MEK2 antibody (Anti-MEK1 + MEK2 antibody [EPR16667] ab178876) loading control staining at 1/1000 dilution.
All lanes: Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] (Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg
Lane 2: NIH/3T3 treated with 100ng/ml nocodazole for 24h whole cell lysate (untreated membrane) at 20 µg
Lane 3: NIH/3T3 treated with 100ng/ml nocodazole for 24h whole cell lysate (phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 82s
![Dot Blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--dot-blot-img408880.jpg "Dot Blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)")
Dot Blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)
This data was produced using Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509, the same clone but in a different formulation.
Dot blot analysis of MEK1 (phospho T286) using Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509 at 1:1000 (0.48 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Exposure time: 18 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lane 1: MEK1 (phospho T286) peptide a
Lane 2: MEK1 (phospho T286) peptide b
Lane 3: MEK1 non-phospho peptide
All lanes: Dot Blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] (Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509) at 1/1000 dilution
Lane 1: MEK1 (phospho T286) peptide a
Lane 2: MEK1 (phospho T286) peptide b
Lane 3: MEK1 non-phospho peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
![Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--immunocytochemistry-immunofluorescence-img408879.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)")
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)
This data was produced using Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling MEK1 (phospho T286) with Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509 at 1/1000 (0.48 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal showing cytoplasmic and nuclear staining in NIH/3T3 cells in M phase.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).![Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--immunocytochemistry-immunofluorescence-img408878.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)")
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)
This data was produced using Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MAP2K1 KO HAP1 (MAP2K1 knockout human chronic myelogenous leukemia near-haploid cell) cells labelling MEK1 (phospho T286) with Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509 at 1/1000 (0.48 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal showing cytoplasmic and nuclear staining in wildtype HAP1 cells in M phase and no staining in MAP2K1 knockout HAP1 cells.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).![Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--western-blot-img408827.jpg "Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)")
Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)
This data was produced using Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509, the same clone but in a different formulation.
Blocking and diluting buffer and concentration: 1% BSA/TBST. The identity of the higher MW bands between 100kDa and 200kDa are unknown.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
In Western blot, anti-MEK1 antibody (Anti-MEK1 antibody [Y77] ab32576) loading control staining at 1/1000 dilution.
Exposure time: 15 seconds.All lanes: Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] (Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509) at 1/1000 dilution
Lane 1: Untreated wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (untreated membrane), at 20 µg
Lane 2: Wild-type HAP1 treated with 100ng/ml nocodazole for 24h whole cell lysate (untreated membrane) at 20 µg
Lane 3: untreated MEK1 knockout HAP1 whole cell lysate at 20 µg
Lane 4: MEK1 knockout HAP1 treated with 100ng/ml nocodazole for 24h whole cell lysate at 20 µg
Lane 5: Untreated wild-type HAP1 whole cell lysate (phosphatase treated membrane), at 20 µg
Lane 6: Wild-type HAP1 treated with 100ng/ml nocodazole for 24h whole cell lysate (phosphatase treated membrane) at 20 µg
Lane 7: Untreated MEK1 knockout HAP1 whole cell lysate (phosphatase treated membrane) at 20 µg
Lane 8: MEK1 knockout HAP1 treated with 100ng/ml nocodazole for 24h whole cell lysate (phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 15s
![Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--western-blot-img408825.jpg "Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)")
Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)
This data was produced using Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509, the same clone but in a different formulation.
Blocking and diluting buffer and concentration: 1% BSA/TBST.
The identity of the higher MW bands between 100kDa and 200kDa are unknown.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
In Western blot, anti-MEK1 antibody (Anti-MEK1 antibody [Y77] ab32576) loading control staining at 1/1000 dilution.
Exposure time: 26 seconds.All lanes: Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] (Anti-MEK1 (phospho T286) antibody [EPR27226-8] ab307509) at 1/1000 dilution
Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg
Lane 2: HeLa treated with 100ng/ml nocodazole for 24h whole cell lysate (untreated membrane) at 20 µg
Lane 3: HeLa treated with 100ng/ml nocodazole for 24h whole cell lysate (phosphatase treated membrane at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 26s
![Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510), expandable thumbnail](https://content.abcam.com/products/images/mek1-phospho-t286-antibody-epr27226-8-bsa-and-azide-free-ab307510--western-blot-img408825.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MEK1 (phospho T286) antibody [EPR27226-8] - BSA and Azide free (ab307510)")
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