Rabbit Recombinant Monoclonal MP2K6 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Images
![Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--western-blot-img107586.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106730.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322)")
![Immunocytochemistry/ Immunofluorescence - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunocytochemistry-immunofluorescence-img127996.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322)")
![Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--western-blot-img46130.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89181.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (AB251322)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Expected |
| Mouse | Expected | Expected | Tested | Expected | Tested |
| Rat | Expected | Expected | Tested | Expected | Expected |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
Additional Targets
MAP2K3
Alternative names
MEK6, MKK6, PRKMK6, SKK3, MAP2K6, Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6, MAPKK 6, MAPK/ERK kinase 6, Stress-activated protein kinase kinase 3, MEK 6, SAPK kinase 3, SAPKK-3, SAPKK3
Recommended products
Rabbit Recombinant Monoclonal MP2K6 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17340
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251322 is the carrier-free version of Anti-MEK3 + MEK6 antibody [EPR17340] ab200831.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEK3 and MEK6 also known as MAP2K3 and MAP2K6 are dual-specificity protein kinases. MEK3 has a mass of approximately 39 kDa while MEK6 is around 37 kDa. They are both part of the MAP kinase family and mainly expressed in tissues like the skeletal muscle liver and heart. These kinases play a role in phosphorylating and activating p38 MAP kinases which are important for intracellular signaling.
- Biological function summary
MEK3 and MEK6 are key components of the MAP kinase signaling pathway. These kinases participate in response networks that regulate stress-induced signaling and inflammatory responses. Although not explicitly forming a classic complex they interact with substrates like p38 MAPK to exert their effects. MEK3 and MEK6 contribute to cellular processes including differentiation apoptosis and gene expression.
- Pathways
MEK3 and MEK6 are integral to the p38 MAPK pathway and the stress-activated protein kinase (SAPK) pathways. These pathways facilitate the cellular response to external stress hypoxia and inflammatory cytokines. Their activity is closely related to other MAP kinases like JNK in propagating signals that control cellular stress responses. Their activation mechanism involves sequential phosphorylation events that link initial stimuli to specific cellular reactions.
- Associated diseases and disorders
MEK3 and MEK6 are implicated in inflammatory diseases and certain types of cancer. For instance abnormal activity of these kinases is observed in rheumatoid arthritis due to their role in inflammation and cellular differentiation. They also have links to cancers like melanoma through their regulation of apoptosis and cell proliferation. The dysregulation of their network with p38 MAPK and JNK can contribute to the pathology of these conditions making them potential targets for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--western-blot-img107586.jpg "Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] (Anti-MEK3 + MEK6 antibody [EPR17340] ab200831) at 1/1000 dilution
Lane 1: Mouse spleen lysate at 10 µg
Lane 2: Rat spleen lysate at 10 µg
Lane 3: C6 (Rat glial tumor cells) whole cell lysate
Lane 4: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 6: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 34 kDa, 37 kDa, 39 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106730.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MEK3 + MEK6 with Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on Mouse liver tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunocytochemistry-immunofluorescence-img127996.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Immunocytochemistry/ Immunofluorescence - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human hepatocellular carcinoma) cells labeling MEK3 + MEK6 with Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Nuclear and cytoplasmic staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--western-blot-img46130.jpg "Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Recombinant fragment of Human MEK6 protein contains aa139-334 with His-Tag®(22kDa).
All lanes: Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] (Anti-MEK3 + MEK6 antibody [EPR17340] ab200831) at 1/10000 dilution
All lanes: Human MEK6 recombinant protein fragment at 10 µg
Predicted band size: 39 kDa
Exposure time: 15s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89181.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling MEK3 + MEK6 with Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--western-blot-img23568.jpg "Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] (Anti-MEK3 + MEK6 antibody [EPR17340] ab200831) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg
Lane 3: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 34 kDa, 37 kDa, 39 kDa
Exposure time: 15s
![Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunoprecipitation-img847.jpg "Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.MEK3 + MEK6 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 at 1/100 dilution. Western blot was performed from the immunoprecipitate using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds.All lanes: Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17340] (Anti-MEK3 + MEK6 antibody [EPR17340] ab200831)
Predicted band size: 39 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54343.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MEK3 + MEK6 with Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on Human spleen tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--western-blot-img145909.jpg "Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] (Anti-MEK3 + MEK6 antibody [EPR17340] ab200831) at 1/10000 dilution
All lanes: Human fetal liver tissue lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 34 kDa, 37 kDa, 39 kDa
Exposure time: 1min
![Flow Cytometry (Intracellular) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--flow-cytometry-intracellular-img27638.jpg "Flow Cytometry (Intracellular) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
Flow Cytometry (Intracellular) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)
This data was developed using Anti-MEK3 + MEK6 antibody [EPR17340] ab200831, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of HeLa cells labelling MEK3 + MEK6 (red) with purified Anti-MEK3 + MEK6 antibody [EPR17340] ab200831 at dilution of 1/150. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
![Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--western-blot-img23568.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
![Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunoprecipitation-img847.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322), expandable thumbnail](https://content.abcam.com/products/images/mek3-mek6-antibody-epr17340-bsa-and-azide-free-ab251322--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54343.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17340] - BSA and Azide free (ab251322)")
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